MP1 Antibody (H-6): sc-376783
- MP1 Antibody (H-6) is a mouse monoclonal IgG1 κ MP1 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-29 at the N-terminus of MP1 of mouse origin
- MP1 Antibody (H-6) is recommended for detection of MP1 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-MP1 Antibody (H-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MP1 Antibody (H-6) for WB and IHC(P) applications. These reagents are now offered in bundles with MP1 Antibody (H-6) (see ordering information below).
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MP1 Antibody (H-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects MP1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MP1 (H-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MP1, also known as MEK partner 1, plays a crucial role as a scaffolding protein within the mitogen-activated protein (MAP) kinase signaling pathway, which is essential for cellular responses to growth factors. By binding to the proline-rich domain of MEK-1, MP1 enhances the phosphorylation of MEK-1 by the activating kinase B-Raf, thereby facilitating the activation of extracellular signal-regulated kinase (ERK) 1. This interaction is vital for the specificity of the MAPK pathway, as MP1 preferentially associates with MEK-1 and ERK 1, while showing no affinity for MEK-2 or ERK 2. Such specificity is important for ensuring that appropriate cellular responses are triggered in response to various extracellular signals, highlighting MP1′s significance in regulating cell growth and differentiation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MP1 Antibody (H-6) References:
- Mitogen-activated protein kinases: specific messages from ubiquitous messengers. | Schaeffer, HJ. and Weber, MJ. 1999. Mol Cell Biol. 19: 2435-44. PMID: 10082509
- Organization and regulation of mitogen-activated protein kinase signaling pathways. | Garrington, TP. and Johnson, GL. 1999. Curr Opin Cell Biol. 11: 211-8. PMID: 10209154
- MEK partner 1 (MP1): regulation of oligomerization in MAP kinase signaling. | Sharma, C., et al. 2005. J Cell Biochem. 94: 708-19. PMID: 15547943
- The MEK1 scaffolding protein MP1 regulates cell spreading by integrating PAK1 and Rho signals. | Pullikuth, A., et al. 2005. Mol Cell Biol. 25: 5119-33. PMID: 15923628
- Involvement of the MP1 scaffold protein in ERK signaling regulation during Drosophila wing development. | Mouchel-Vielh, E., et al. 2008. Genes Cells. 13: 1099-111. PMID: 18823331
- MP1: a MEK binding partner that enhances enzymatic activation of the MAP kinase cascade. | Schaeffer, HJ., et al. 1998. Science. 281: 1668-71. PMID: 9733512
- A mammalian scaffold complex that selectively mediates MAP kinase activation. | Whitmarsh, AJ., et al. 1998. Science. 281: 1671-4. PMID: 9733513
- Routing MAP kinase cascades. | Elion, EA. 1998. Science. 281: 1625-6. PMID: 9767029
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MP1 Antibody (H-6) | sc-376783 | 200 µg/ml | $322.00 | |||
MP1 Antibody (H-6): m-IgGκ BP-HRP Bundle | sc-523090 | 200 µg Ab, 40 µg BP | $361.00 | |||
MP1 Antibody (H-6): m-IgG1 BP-HRP Bundle | sc-533551 | 200 µg Ab; 20 µg BP | $361.00 | |||
MP1 Antibody (H-6) AC | sc-376783 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MP1 Antibody (H-6) HRP | sc-376783 HRP | 200 µg/ml | $322.00 | |||
MP1 Antibody (H-6) FITC | sc-376783 FITC | 200 µg/ml | $336.00 | |||
MP1 Antibody (H-6) PE | sc-376783 PE | 200 µg/ml | $349.00 | |||
MP1 Antibody (H-6) Alexa Fluor® 488 | sc-376783 AF488 | 200 µg/ml | $364.00 | |||
MP1 Antibody (H-6) Alexa Fluor® 546 | sc-376783 AF546 | 200 µg/ml | $364.00 | |||
MP1 Antibody (H-6) Alexa Fluor® 594 | sc-376783 AF594 | 200 µg/ml | $364.00 | |||
MP1 Antibody (H-6) Alexa Fluor® 647 | sc-376783 AF647 | 200 µg/ml | $364.00 | |||
MP1 Antibody (H-6) Alexa Fluor® 680 | sc-376783 AF680 | 200 µg/ml | $364.00 | |||
MP1 Antibody (H-6) Alexa Fluor® 790 | sc-376783 AF790 | 200 µg/ml | $364.00 | |||
MP1 (H-6) Neutralizing Peptide | sc-376783 P | 100 µg/0.5 ml | $69.00 |