Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Mox1 Double Nickase Plasmid (m): sc-433531-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mox1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Mox1 Double Nickase Plasmid (m) and Mox1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Nox1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mox1 Antibody (C-10): sc-518023
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mox1 Double Nickase Plasmid (m) | sc-433531-NIC | 20 µg | $410.00 | |||
Mox1 Double Nickase Plasmid (m2) | sc-433531-NIC-2 | 20 µg | $410.00 |
Mouse Nox1 encodes the NADPH oxidase isoform NOX1 (also referred to as Mox1), a membrane-associated enzyme that generates reactive oxygen species and shapes redox-dependent signaling. NOX1-derived oxidants modulate pathways linked to proliferation, migration, cytoskeletal remodeling, and inflammatory transcriptional programs, including MAPK and NF-κB signaling, with effects on epithelial and vascular cell biology. In experimental models, altered NOX1 activity is frequently connected to oxidative stress phenotypes and changes in host-defense and barrier functions. These properties make Nox1 a useful target for dissecting ROS-regulated signal transduction and redox control of gene expression.
Mox1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Nox1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Nox1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Nox1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Nox1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.