Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MOR-1 CRISPR/Cas9 KO Plasmid (h): sc-401224
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MOR-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MOR-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MOR-1 Antibody (D-12): sc-515933
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MOR-1 CRISPR/Cas9 KO Plasmid (h) | sc-401224 | 20 µg | $397.00 |
Overview
OPRM1 encodes the human μ-opioid receptor MOR-1, a Gi/Go-coupled GPCR that binds endogenous opioid peptides and exogenous opioid ligands to regulate neuronal excitability and neurotransmitter release. MOR-1 signaling inhibits adenylyl cyclase, modulates cAMP/PKA pathways, engages MAPK/ERK cascades, and influences ion channel activity, thereby shaping synaptic transmission in pain, reward, and stress circuits. Receptor activation drives β-arrestin recruitment, desensitization, and trafficking, linking MOR-1 to receptor internalization and adaptive signaling responses. Altered OPRM1 function or regulation has been associated with interindividual differences in analgesic response, substance use phenotypes, and neuropsychiatric traits, making it relevant for mechanistic studies in neurobiology and pharmacology.
MOR-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the OPRM1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the OPRM1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the OPRM1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MOR-1 protein expression.
This CRISPR knockout system enables efficient generation of OPRM1-deficient cell models for investigation of MOR-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting OPRM1 exon(s) critical for MOR-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple OPRM1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MOR-1 CRISPR/Cas9 KO Plasmid (h) and MOR-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the OPRM1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MOR-1 HDR Plasmid (h) and MOR-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by OPRM1 homology arms to support homology-directed repair at defined OPRM1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.