MondoA Antibody (5F3): sc-293403

MondoA Antibody (5F3) is a mouse monoclonal IgG2a kappa light chain antibody that detects MondoA protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-MondoA antibody (5F3) is available as a non-conjugated format. MondoA, also known as MLXIP (MLX interacting protein), KIAA0867, or MIR, is a 919 amino acid protein that plays a crucial role in cellular metabolism and gene regulation. MondoA is primarily localized in the nucleus and cytoplasm, as well as the outer mitochondrial membrane, where MondoA is involved in the regulation of glucose-responsive genes. MondoA contains a basic helix-loop-helix (bHLH) domain, which is essential for function as a transcription factor that binds to DNA at the canonical E box sequence 5′-CACGTG-3′. This binding is vital for the transcriptional activation of genes involved in glucose metabolism, making MondoA a key player in energy homeostasis and metabolic disorders. MondoA is expressed in various tissues, with the highest levels found in skeletal muscle, highlighting MondoA′s importance in muscle metabolism. The gene encoding MondoA is located on human chromosome 12, which is associated with numerous genetic disorders, including hypochondrogenesis and Noonan syndrome, underscoring MondoA′s significance in both health and disease.

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MondoA Antibody (5F3) References:

1. Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.  |  Nagase, T., et al. 1998. DNA Res. 5: 355-64. PMID: 10048485
2. MondoA, a novel basic helix-loop-helix-leucine zipper transcriptional activator that constitutes a positive branch of a max-like network.  |  Billin, AN., et al. 2000. Mol Cell Biol. 20: 8845-54. PMID: 11073985
3. A novel heterodimerization domain, CRM1, and 14-3-3 control subcellular localization of the MondoA-Mlx heterocomplex.  |  Eilers, AL., et al. 2002. Mol Cell Biol. 22: 8514-26. PMID: 12446771
4. MSAP is a novel MIR-interacting protein that enhances neurite outgrowth and increases myosin regulatory light chain.  |  Bornhauser, BC., et al. 2003. J Biol Chem. 278: 35412-20. PMID: 12826659
5. MondoA-Mlx heterodimers are candidate sensors of cellular energy status: mitochondrial localization and direct regulation of glycolysis.  |  Sans, CL., et al. 2006. Mol Cell Biol. 26: 4863-71. PMID: 16782875
6. Glucose sensing by MondoA:Mlx complexes: a role for hexokinases and direct regulation of thioredoxin-interacting protein expression.  |  Stoltzman, CA., et al. 2008. Proc Natl Acad Sci U S A. 105: 6912-7. PMID: 18458340