MOAP1 Double Nickase Plasmid (h): sc-408271-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MOAP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MOAP1 Double Nickase Plasmid (h) and MOAP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MOAP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MOAP1 Antibody (A-11): sc-271338
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MOAP1 Double Nickase Plasmid (h) | sc-408271-NIC | 20 µg | $410.00 | |||
MOAP1 Double Nickase Plasmid (h2) | sc-408271-NIC-2 | 20 µg | $410.00 |
MOAP1 (modulator of apoptosis 1) encodes a mitochondrial outer membrane–associated protein that functions as a pro-apoptotic regulator by coupling upstream stress signals to BAX activation and mitochondrial outer membrane permeabilization. Through interactions with factors such as RASSF1A and BAX, MOAP1 influences intrinsic apoptosis, caspase activation, and mitochondrial dynamics, linking cell fate decisions to damage and checkpoint pathways. Altered MOAP1 expression or regulation has been associated with impaired apoptotic competence, a feature relevant to tumor biology and cellular responses to genotoxic stress. As a node connecting mitochondrial signaling and programmed cell death, MOAP1 is commonly studied in models of oncogenesis, neurodegeneration, and stress-induced cytotoxicity.
MOAP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MOAP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MOAP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MOAP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MOAP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.