Mnk1 HDR Plasmid (h): sc-402417-HDR. Western blot analysis of Mnk1 expression in non-transfected control (A) and puromycin treated, Mnk1 CRISPR/Cas9 KO Plasmid: sc-402417 plus Mnk1 HDR Plasmid co-transfected (B) HEK 293T whole cell lysates. Blot probed with Mnk1 (C-20): sc-6965. β-Actin (C4): sc-47778 used as specificity and loading control.
Mnk1 CRISPR/Cas9 KO Plasmid (h): sc-402417
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mnk1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Mnk1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- Mnk1 HDR Plasmid (h) (sc-402417-HDR) is recommended for co-transfection with Mnk1 CRISPR/Cas9 KO Plasmid (h) to enable selection of successfully edited cells through HDR-mediated integration of a puromycin resistance cassette and RFP reporter gene
- Mnk1 HDR Plasmid (h) is a pool of plasmids, each containing a homology-directed repair (HDR) template corresponding to the gRNA target sites in the Mnk1 CRISPR/Cas9 KO Plasmid (h)
- Each HDR plasmid contains two ~800 bp homology arms flanking the puromycin resistance and RFP cassettes, designed to bind genomic DNA sequences surrounding the Cas9-induced double-strand break site and facilitate precise HDR-mediated integration
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mnk1 Antibody (A-4): sc-133107
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mnk1 CRISPR/Cas9 KO Plasmid (h) | sc-402417 | 20 µg | $397.00 | |||
Mnk1 HDR Plasmid (h) | sc-402417-HDR | 20 µg | $445.00 |