Mnk1 Antibody (A-4): sc-133107

Mnk1 Antibody (A-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Mnk1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Mnk1 antibody (A-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Mnk1 plays a crucial role in cellular signaling pathways, particularly in regulating protein synthesis in response to stress signals. Mnk1 is primarily located in the cytoplasm, where Mnk1 interacts with eukaryotic translation initiation factor 4E (eIF4E), a key player in translation initiation. This interaction enables eIF4E phosphorylation, which enhances affinity for the mRNA cap structure, promoting translation of specific mRNAs critical for cell survival and proliferation under stress conditions. Mnk1′s ability to modulate protein synthesis in response to environmental cues underscores Mnk1′s importance in various physiological processes, including cell growth, differentiation, and stress response, making Mnk1 a significant target for research in cancer and other diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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Mnk1 Antibody (A-4) References:

1. MAPKAP kinase-2; a novel protein kinase activated by mitogen-activated protein kinase.  |  Stokoe, D., et al. 1992. EMBO J. 11: 3985-94. PMID: 1327754
2. Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.  |  Sturgill, TW., et al. 1988. Nature. 334: 715-8. PMID: 2842685
3. Role of MAPK/MNK1 signaling in virus replication.  |  Kumar, R., et al. 2018. Virus Res. 253: 48-61. PMID: 29864503
4. The MNK1/2-eIF4E Axis as a Potential Therapeutic Target in Melanoma.  |  Prabhu, SA., et al. 2020. Int J Mol Sci. 21: PMID: 32517051
5. Mnk1/2 kinases regulate memory and autism-related behaviours via Syngap1.  |  Chalkiadaki, K., et al. 2023. Brain. 146: 2175-2190. PMID: 36315645
6. MNK1/2 inhibition limits oncogenicity and metastasis of KIT-mutant melanoma.  |  Zhan, Y., et al. 2024. J Clin Invest. 134: PMID: 38618965
7. RSK3 encodes a novel pp90rsk isoform with a unique N-terminal sequence: growth factor-stimulated kinase function and nuclear translocation.  |  Zhao, Y., et al. 1995. Mol Cell Biol. 15: 4353-63. PMID: 7623830
8. The mitogen-activated protein kinase signal transduction pathway.  |  Davis, RJ. 1993. J Biol Chem. 268: 14553-6. PMID: 8325833
9. 3pK, a new mitogen-activated protein kinase-activated protein kinase located in the small cell lung cancer tumor suppressor gene region.  |  Sithanandam, G., et al. 1996. Mol Cell Biol. 16: 868-76. PMID: 8622688
10. Identification of mitogen-activated protein (MAP) kinase-activated protein kinase-3, a novel substrate of CSBP p38 MAP kinase.  |  McLaughlin, MM., et al. 1996. J Biol Chem. 271: 8488-92. PMID: 8626550
11. Mitogen-activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2.  |  Waskiewicz, AJ., et al. 1997. EMBO J. 16: 1909-20. PMID: 9155017
12. MNK1, a new MAP kinase-activated protein kinase, isolated by a novel expression screening method for identifying protein kinase substrates.  |  Fukunaga, R. and Hunter, T. 1997. EMBO J. 16: 1921-33. PMID: 9155018

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mnk1 Antibody (A-4) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Mnk1 Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mnk1 Antibody (A-4) Specifications

Alternate Product Name: Mnk1

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 411-465

Epitope Target: Mnk1

Epitope Species: human

Product Reactivity/Detection: Mnk1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Mnk1 Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin