MMP9 (h2): 293T Lysate: sc-176046

MMP-9 (h2): 293T Lysate, derived from the HEK 293T cell line, is instrumental in studying the function and regulation of Matrix Metalloproteinase-9 (MMP-9) in human cells. The 293T cells, a variant of the HEK 293 line, have been engineered to express the SV40 large T antigen, enhancing their protein production capabilities, making them suitable for detailed biochemical analyses. MMP-9, also known as gelatinase B, is crucial in degrading components of the extracellular matrix (ECM), such as collagen and gelatin, thereby playing a vital role in tissue remodeling and cellular migration. Researchers use this lysate to investigate the enzymatic activity and regulatory mechanisms of MMP-9, employing techniques such as zymography, Western blotting, and enzyme-linked immunosorbent assays (ELISAs) to quantify and analyze MMP-9 expression and activity. These studies focus on understanding how MMP-9 is involved in processes like cell migration, invasion, and ECM remodeling. By exploring these mechanisms, scientists gain insights into the fundamental aspects of cellular behavior and tissue dynamics, providing a deeper understanding of MMP-9's role in various physiological processes, strictly within the context of basic scientific research.