MMP-20 CRISPR/Cas9 KO Plasmid (h): sc-405838
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MMP-20 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MMP-20 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MMP-20 CRISPR/Cas9 KO Plasmid (h) | sc-405838 | 20 µg | $397.00 |
Overview
MMP20 encodes matrix metalloproteinase-20 (MMP-20, enamelysin), a zinc-dependent endopeptidase specialized for extracellular matrix remodeling with prominent activity during enamel formation. By cleaving structural matrix proteins and processing ECM components, MMP-20 contributes to regulated proteolysis that coordinates tissue maturation and mineralization processes. Its activity interfaces with broader metalloproteinase-regulated pathways that shape cell–matrix interactions, protease activation cascades, and extracellular signaling dynamics. Dysregulated MMP20 function is associated with defects in enamel development and has been examined in the context of proteolysis-driven changes in tissue microenvironments relevant to disease biology.
MMP-20 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MMP20 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MMP20 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MMP20 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MMP-20 protein expression.
This CRISPR knockout system enables efficient generation of MMP20-deficient cell models for investigation of MMP-20 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MMP20 exon(s) critical for MMP-20 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MMP20 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MMP-20 CRISPR/Cas9 KO Plasmid (h) and MMP-20 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MMP20 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MMP-20 HDR Plasmid (h) and MMP-20 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MMP20 homology arms to support homology-directed repair at defined MMP20 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.