MMP-1 Antibody (3B6): sc-21731

MMP-1 Antibody (3B6) is a mouse monoclonal IgG1 antibody designed to detect human matrix metalloproteinase-1 (MMP-1) protein, also known as interstitial collagenase, fibroblast collagenase, or collagenase-1. MMP-1 monoclonal antibody (3B6) targets the amino acid region 366-376 of human MMP-1 protein, ensuring precise binding and reliable detection across various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MMP-1 plays a critical role in degrading extracellular matrix components such as collagen, gelatin, fibronectin, laminin, and proteoglycans, which is essential for processes like tissue remodeling, wound healing, and angiogenesis. By breaking down these matrix proteins, MMP-1 facilitates cellular migration and invasion, making MMP-1 a key player in both normal physiological and pathological conditions, including cancer metastasis and rheumatoid arthritis. The regulation of MMP-1 is intricately linked to cellular signaling pathways, where p53 downregulates MMP-1, highlighting MMP-1′s importance in maintaining cellular homeostasis and preventing excessive tissue degradation. MMP-1 requires calcium and zinc ions for catalytic activity, which is characteristic of the matrix metalloproteinase family. Additionally, MMP-1 works in concert with other metalloproteinases, such as MMP-9 (gelatinase B), to degrade bone collagens and may contribute to osteoclastic bone resorption. Anti-MMP-1 antibody (3B6) is available in both non-conjugated and various conjugated forms, including agarose and multiple Alexa Fluor® conjugates, providing flexibility for diverse experimental needs.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MMP-1 Antibody (3B6) References:

1. p53 down-regulates human matrix metalloproteinase-1 (Collagenase-1) gene expression.  |  Sun, Y., et al. 1999. J Biol Chem. 274: 11535-40. PMID: 10206959
2. Cloning and characterization of human tumor cell interstitial collagenase.  |  Templeton, NS., et al. 1990. Cancer Res. 50: 5431-7. PMID: 2167156
3. Localization of matrix metalloproteinase 9 (92-kilodalton gelatinase/type IV collagenase = gelatinase B) in osteoclasts: implications for bone resorption.  |  Okada, Y., et al. 1995. Lab Invest. 72: 311-22. PMID: 7898050
4. High expression of 92-kD type IV collagenase (gelatinase B) in the osteoclast lineage during mouse development.  |  Reponen, P., et al. 1994. J Cell Biol. 124: 1091-1102. PMID: 8132709
5. Structural implications for the role of the N terminus in the 'superactivation' of collagenases. A crystallographic study.  |  Reinemer, P., et al. 1994. FEBS Lett. 338: 227-33. PMID: 8307185
6. Matrix metalloproteinases: a review.  |  Birkedal-Hansen, H., et al. 1993. Crit Rev Oral Biol Med. 4: 197-250. PMID: 8435466
7. Expression of several matrix metalloproteinase genes in human monocytic cells.  |  Machein, U. and Conca, W. 1997. Adv Exp Med Biol. 421: 247-51. PMID: 9330704

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MMP-1 Antibody (3B6) has 97 citations in a variety of scientific publications.

Primary antibodies like Anti-MMP-1 Antibody (3B6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MMP-1 Antibody (3B6) Specifications

Alternate Product Name: MMP-1

Product Clone Name: 3B6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 366-376

Epitope Target: MMP-1

Epitope Species: human

Product Reactivity/Detection: MMP-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 97 citations in various scientific publications

Available Conjugates/Forms for MMP-1 Antibody (3B6): AC: Agarose, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790

The preferred MMP-1 antibody is MMP-1 Antibody (SB12e): sc-58377.