MLSN1 Antibody (F-3): sc-515228

MLSN1 Antibody (F-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects MLSN1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MLSN1 (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MLSN1, also known as melastatin, is a 1,533 amino acid protein that plays a crucial role in regulating calcium ion transport across cell membranes, as part of the transient receptor potential (Trp) calcium channel family. This function is particularly important in melanocytes, where proper calcium signaling is essential for maintaining normal cellular functions and preventing uncontrolled proliferation. MLSN1 expression is inversely correlated with the proliferation of melanocytic tumors, indicating that adequate levels can inhibit aggressive growth of malignant melanoma. Downregulation of MLSN1 transcripts in malignant melanomas, including primary cutaneous tumors, serves as a significant marker for metastasis, often occurring independently of tumor thickness. anti-MLSN1 antibody (F-3) is a valuable tool for researchers studying melanoma biology and underlying mechanisms of tumor progression.

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MLSN1 Antibody (F-3) References:

1. Identification and characterization of MTR1, a novel gene with homology to melastatin (MLSN1) and the trp gene family located in the BWS-WT2 critical region on chromosome 11p15.5 and showing allele-specific expression.  |  Prawitt, D., et al. 2000. Hum Mol Genet. 9: 203-16. PMID: 10607831
2. Patterns of melastatin mRNA expression in melanocytic tumors.  |  Deeds, J., et al. 2000. Hum Pathol. 31: 1346-56. PMID: 11112208
3. Expression and Up-regulation of alternatively spliced transcripts of melastatin, a melanoma metastasis-related gene, in human melanoma cells.  |  Fang, D. and Setaluri, V. 2000. Biochem Biophys Res Commun. 279: 53-61. PMID: 11112417
4. Melastatin expression and prognosis in cutaneous malignant melanoma.  |  Duncan, LM., et al. 2001. J Clin Oncol. 19: 568-76. PMID: 11208852
5. Frameshift mutations at mononucleotide repeats in RAD50 recombinational DNA repair gene in colorectal cancers with microsatellite instability.  |  Ikenoue, T., et al. 2001. Jpn J Cancer Res. 92: 587-91. PMID: 11429044
6. Human melastatin 1 (TRPM1) is regulated by MITF and produces multiple polypeptide isoforms in melanocytes and melanoma.  |  Zhiqi, S., et al. 2004. Melanoma Res. 14: 509-16. PMID: 15577322
7. Magnesium in man: implications for health and disease.  |  de Baaij, JH., et al. 2015. Physiol Rev. 95: 1-46. PMID: 25540137
8. Heterogeneity of Metastatic Melanoma:  Correlation of MITF With Its Transcriptional Targets MLSN1, PEDF, HMB-45, and MART-1.  |  Zand, S., et al. 2016. Am J Clin Pathol. 146: 353-60. PMID: 27515936
9. TRP (transient receptor potential) ion channel family: structures, biological functions and therapeutic interventions for diseases.  |  Zhang, M., et al. 2023. Signal Transduct Target Ther. 8: 261. PMID: 37402746
10. TRPM channels in health and disease.  |  Chubanov, V., et al. 2024. Nat Rev Nephrol. 20: 175-187. PMID: 37853091
11. Down-regulation of the novel gene melastatin correlates with potential for melanoma metastasis.  |  Duncan, LM., et al. 1998. Cancer Res. 58: 1515-20. PMID: 9537257
12. Chromosomal localization and genomic characterization of the mouse melastatin gene (Mlsn1).  |  Hunter, JJ., et al. 1998. Genomics. 54: 116-23. PMID: 9806836

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MLSN1 Antibody (F-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-MLSN1 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MLSN1 Antibody (F-3) Specifications

Alternate Product Name: MLSN1

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1304-1603

Epitope Target: MLSN1

Epitope Species: human

Product Reactivity/Detection: MLSN1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for MLSN1 Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin