MKP-6 Antibody (4B5-E6): sc-517023

MKP-6 Antibody (4B5-E6) is a mouse monoclonal IgG2a kappa light chain antibody that detects MKP-6 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MKP-6 antibody (4B5-E6) is available as the non-conjugated format. MAP kinase phosphatase 6 (MKP-6), also known as dual-specificity phosphatase 14 (DUSP14), is a 198 amino acid protein that plays a crucial role in cellular signaling by dephosphorylating key mitogen-activated protein kinases (MAPKs) such as ERK, JNK, and p38, thereby acting as a negative regulator of CD28 signaling in T cells. This regulation is particularly important for maintaining immune homeostasis and preventing overactivation of T cells, which can lead to autoimmune diseases. MKP-6 is characterized by a conserved catalytic core typical of the MKP family, but also possesses unique N- and C-terminal regions that distinguish MKP-6 from other members of the family. Notably, MKP-6 interacts specifically with the cytoplasmic tail of CD28 at Tyr 200, a critical interaction for T cell activation, although this residue can be mutated to Phe 200 without affecting binding. MKP-6 is expressed ubiquitously across various tissues, with higher expression levels observed in certain cell types, underscoring its significance in diverse biological processes.

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MKP-6 Antibody (4B5-E6) References:

1. Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6.  |  Marti, F., et al. 2001. J Immunol. 166: 197-206. PMID: 11123293
2. A Novel MAPK phosphatase MKP-7 acts preferentially on JNK/SAPK and p38 alpha and beta MAPKs.  |  Tanoue, T., et al. 2001. J Biol Chem. 276: 26629-39. PMID: 11359773
3. Ataxia telangiectasia mutated down-regulates phospho-extracellular signal-regulated kinase 1/2 via activation of MKP-1 in response to radiation.  |  Nyati, MK., et al. 2006. Cancer Res. 66: 11554-9. PMID: 17178844
4. Novel function of DUSP14/MKP6 (dual specific phosphatase 14) as a nonspecific regulatory molecule for delayed-type hypersensitivity.  |  Nakano, Y. 2007. Br J Dermatol. 156: 848-60. PMID: 17263825
5. Increasing GLP-1-induced beta-cell proliferation by silencing the negative regulators of signaling cAMP response element modulator-alpha and DUSP14.  |  Klinger, S., et al. 2008. Diabetes. 57: 584-93. PMID: 18025410
6. Mycobacterial lipomannan induces MAP kinase phosphatase-1 expression in macrophages.  |  Elass, E., et al. 2008. FEBS Lett. 582: 445-50. PMID: 18201568

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MKP-6 Antibody (4B5-E6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-MKP-6 Antibody (4B5-E6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MKP-6 Antibody (4B5-E6) Specifications

Alternate Product Name: MKP-6

Product Clone Name: 4B5-E6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-198

Epitope Target: MKP-6

Epitope Species: human

Product Reactivity/Detection: MKP-6

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for MKP-6 Antibody (4B5-E6): N/A