MKP-5 Antibody (G-10): sc-374276
- MKP-5 Antibody (G-10) is a mouse monoclonal IgG2a κ MKP-5 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-205 mapping at the N-terminus of MKP-5 of human origin
- MKP-5 Antibody (G-10) is recommended for detection of MKP-5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-MKP-5 Antibody (G-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for MKP-5 Antibody (G-10) for WB and IHC(P) applications. These reagents are now offered in bundles with MKP-5 Antibody (G-10) (see ordering information below).
MKP-5 Antibody (G-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects MKP-5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MKP-5 (G-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MKP-5 protein, also known as dual specificity phosphatase 10 (DUSP10), plays a crucial role in regulating mitogen-activated protein (MAP) kinase signaling pathways, which are essential for mediating cellular responses to a variety of stimuli. Specifically, MKP-5 dephosphorylates and inactivates p38 MAPK and SAPK/JNK, thereby modulating processes such as cell proliferation, apoptosis, and immune responses. MKP-5′s ability to regulate these pathways is vital for maintaining cellular homeostasis and preventing excessive inflammatory responses, which can lead to pathological conditions. MKP-5 is ubiquitously expressed and can be found in both cytoplasm and nucleus, highlighting MKP-5′s importance in various cellular contexts. Using anti-MKP-5 antibody (G-10) in research can provide valuable insights into intricate signaling mechanisms that govern cellular behavior and contribute to disease progression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MKP-5 Antibody (G-10) References:
- Molecular cloning and characterization of a novel dual specificity phosphatase, MKP-5. | Tanoue, T., et al. 1999. J Biol Chem. 274: 19949-56. PMID: 10391943
- MKP5, a new member of the MAP kinase phosphatase family, which selectively dephosphorylates stress-activated kinases. | Theodosiou, A., et al. 1999. Oncogene. 18: 6981-8. PMID: 10597297
- Dual specificity phosphatases: a gene family for control of MAP kinase function. | Camps, M., et al. 2000. FASEB J. 14: 6-16. PMID: 10627275
- Expression and comparative chromosomal mapping of MKP-5 genes DUSP10/Dusp10. | Masuda, K., et al. 2000. Cytogenet Cell Genet. 90: 71-4. PMID: 11060451
- ATM-dependent activation of the gene encoding MAP kinase phosphatase 5 by radiomimetic DNA damage. | Bar-Shira, A., et al. 2002. Oncogene. 21: 849-55. PMID: 11850813
- Regulation of innate and adaptive immune responses by MAP kinase phosphatase 5. | Zhang, Y., et al. 2004. Nature. 430: 793-7. PMID: 15306813
- ERK1/2 and p38 pathways are required for P2Y receptor-mediated prostate cancer invasion. | Chen, L., et al. 2004. Cancer Lett. 215: 239-47. PMID: 15488643
- An emerging family of dual specificity MAP kinase phosphatases. | Keyse, SM. 1995. Biochim Biophys Acta. 1265: 152-60. PMID: 7696343
- Functional studies of dual-specificity phosphatases. | Sun, H. 1998. Methods Mol Biol. 84: 307-18. PMID: 9666459
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MKP-5 Antibody (G-10) | sc-374276 | 200 µg/ml | $316.00 | |||
MKP-5 Antibody (G-10): m-IgG Fc BP-HRP Bundle | sc-525947 | 200 µg Ab; 10 µg BP | $354.00 | |||
MKP-5 Antibody (G-10): m-IgG2a BP-HRP Bundle | sc-546467 | 200 µg Ab; 10 µg BP | $354.00 | |||
MKP-5 Antibody (G-10) AC | sc-374276 AC | 500 µg/ml, 25% agarose | $416.00 | |||
MKP-5 Antibody (G-10) HRP | sc-374276 HRP | 200 µg/ml | $316.00 | |||
MKP-5 Antibody (G-10) FITC | sc-374276 FITC | 200 µg/ml | $330.00 | |||
MKP-5 Antibody (G-10) PE | sc-374276 PE | 200 µg/ml | $343.00 | |||
MKP-5 Antibody (G-10) Alexa Fluor® 488 | sc-374276 AF488 | 200 µg/ml | $357.00 | |||
MKP-5 Antibody (G-10) Alexa Fluor® 546 | sc-374276 AF546 | 200 µg/ml | $357.00 | |||
MKP-5 Antibody (G-10) Alexa Fluor® 594 | sc-374276 AF594 | 200 µg/ml | $357.00 | |||
MKP-5 Antibody (G-10) Alexa Fluor® 647 | sc-374276 AF647 | 200 µg/ml | $357.00 | |||
MKP-5 Antibody (G-10) Alexa Fluor® 680 | sc-374276 AF680 | 200 µg/ml | $357.00 | |||
MKP-5 Antibody (G-10) Alexa Fluor® 790 | sc-374276 AF790 | 200 µg/ml | $357.00 |