Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MKP-4 CRISPR/Cas9 KO Plasmid (h): sc-405872
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MKP-4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MKP-4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MKP-4 Antibody (D-3): sc-137010
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MKP-4 CRISPR/Cas9 KO Plasmid (h) | sc-405872 | 20 µg | $397.00 |
Overview
DUSP9 encodes dual specificity phosphatase 9 (MKP-4), a MAPK phosphatase that dephosphorylates threonine and tyrosine residues on activated MAP kinases to attenuate signaling output. MKP-4 is implicated in negative feedback control of ERK and p38 cascades, influencing cellular proliferation, differentiation, and stress-responsive transcriptional programs. By constraining MAPK pathway duration and amplitude, DUSP9 contributes to regulation of inflammatory signaling and cell fate decisions in multiple tissue contexts. Dysregulated DUSP9/MKP-4 activity has been associated with altered MAPK signaling phenotypes observed in cancer biology, metabolic regulation, and inflammatory disease models, making it relevant for mechanistic pathway studies.
MKP-4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DUSP9 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DUSP9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DUSP9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MKP-4 protein expression.
This CRISPR knockout system enables efficient generation of DUSP9-deficient cell models for investigation of MKP-4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DUSP9 exon(s) critical for MKP-4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DUSP9 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MKP-4 CRISPR/Cas9 KO Plasmid (h) and MKP-4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DUSP9 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MKP-4 HDR Plasmid (h) and MKP-4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DUSP9 homology arms to support homology-directed repair at defined DUSP9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.