MKP-2 Antibody (48): sc-135991
- MKP-2 Antibody (48) is a mouse monoclonal IgG1, cited in 2 publications, provided at 50 µg/0.5 ml
- raised against amino acids 13-127 of MKP-2 of rat origin
- recommended for detection of MKP-2 of mouse, rat and human origin by WB, IP and IF; not recommended for IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MKP-2 Antibody (48). This work is in progress.
MKP-2 Antibody (48) is a mouse monoclonal IgG1 antibody that detects MKP-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). MKP-2, also known as MAP kinase phosphatase 2 or Dual specificity protein phosphatase 4 (DUSP4), plays a critical role in regulating mitogenic signal transduction by dephosphorylating both threonine and tyrosine residues on MAP kinases ERK 1 and ERK 2, which are pivotal in cell proliferation and differentiation. Precise regulation of these MAP kinases is essential for maintaining cellular homeostasis and preventing uncontrolled cell growth, making MKP-2 a significant player in cancer biology. Additionally, MKP-2 is transcriptionally regulated by E2F-1 transcription factor, known for mediating apoptosis and suppressing tumorigenesis. E2F-1 binds to a specific palindromic motif in the MKP-2 promoter, enhancing expression and thereby influencing apoptotic pathways. The interaction between MKP-2 and E2F-1 underscores the potential of targeting this pathway for innovative cancer therapies, as manipulating MKP-2 levels could provide new avenues for treatment strategies aimed at enhancing apoptosis in cancer cells.
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MKP-2 Antibody (48) References:
- The carboxyl-terminal domains of MKP-1 and MKP-2 have inhibitory effects on their phosphatase activity. | Hutter, D., et al. 2002. Mol Cell Biochem. 233: 107-17. PMID: 12083364
- Overexpression of mitogen-activated protein kinase phosphatases MKP1, MKP2 in human breast cancer. | Wang, HY., et al. 2003. Cancer Lett. 191: 229-37. PMID: 12618338
- Arabidopsis MAPK phosphatase 2 (MKP2) positively regulates oxidative stress tolerance and inactivates the MPK3 and MPK6 MAPKs. | Lee, JS. and Ellis, BE. 2007. J Biol Chem. 282: 25020-9. PMID: 17586809
- MAPK phosphatase MKP2 mediates disease responses in Arabidopsis and functionally interacts with MPK3 and MPK6. | Lumbreras, V., et al. 2010. Plant J. 63: 1017-30. PMID: 20626661
- Post-translational regulation of mitogen-activated protein kinase phosphatase-2 (MKP-2) by ERK. | Peng, DJ., et al. 2010. Cell Cycle. 9: 4650-5. PMID: 21084841
- Deletion of the dual specific phosphatase-4 (DUSP-4) gene reveals an essential non-redundant role for MAP kinase phosphatase-2 (MKP-2) in proliferation and cell survival. | Lawan, A., et al. 2011. J Biol Chem. 286: 12933-43. PMID: 21317287
- MAPK phosphatase-2 (MKP-2) is induced by hCG and plays a role in the regulation of CYP11A1 expression in MA-10 Leydig cells. | Gómez, NV., et al. 2013. Endocrinology. 154: 1488-500. PMID: 23471219
- Post-translational regulation of mitogen-activated protein kinase phosphatase (MKP)-1 and MKP-2 in macrophages following lipopolysaccharide stimulation: the role of the C termini of the phosphatases in determining their stability. | Crowell, S., et al. 2014. J Biol Chem. 289: 28753-64. PMID: 25204653
- Whole Genome Microarray Analysis of DUSP4-Deletion Reveals A Novel Role for MAP Kinase Phosphatase-2 (MKP-2) in Macrophage Gene Expression and Function. | Neamatallah, T., et al. 2019. Int J Mol Sci. 20: PMID: 31336892
- Estradiol inhibits fMLP-induced neutrophil migration and superoxide production by upregulating MKP-2 and dephosphorylating ERK. | Zhang, P., et al. 2019. Int Immunopharmacol. 75: 105787. PMID: 31401382
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MKP-2 Antibody (48) | sc-135991 | 50 µg/0.5 ml | $322.00 |