MK Antibody (A-9): sc-46701

MK Antibody (A-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects MK protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-MK antibody (A-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Midkine, or MK, is a heparin-binding growth factor that plays a crucial role in regulating growth and differentiation during embryogenesis, making MK essential for proper development. MK protein is primarily located in the extracellular matrix and is secreted by various cell types, which facilitates cell signaling and interaction with other growth factors. MK expression is tightly controlled during embryonic development, influenced by steroid receptors of the retinoic acid superfamily, and MK′s mature form consists of 118 amino acids with five intrachain disulfide bonds. Notably, MK′s carboxy-terminus harbors the principal heparin-binding site and neurite-promoting sequences, both vital for neurotrophic properties. Additionally, MK has been implicated in various pathological conditions, including an association with colon adenocarcinoma in families with familial polyposis, and MK enhances plasminogen activator activity, underscoring MK′s importance in both developmental biology and disease processes.

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MK Antibody (A-9) References:

1. Genetic variations of the midkine (MK) gene in human sporadic colorectal and gastric cancers.  |  Ahmed, KM., et al. 2000. Int J Mol Med. 6: 281-7. PMID: 10934290
2. A new family of heparin-binding factors: strong conservation of midkine (MK) sequences between the human and the mouse.  |  Tsutsui, J., et al. 1991. Biochem Biophys Res Commun. 176: 792-7. PMID: 2025291
3. Cloning and expression of a developmentally regulated protein that induces mitogenic and neurite outgrowth activity.  |  Li, YS., et al. 1990. Science. 250: 1690-4. PMID: 2270483
4. Midkine rewires the melanoma microenvironment toward a tolerogenic and immune-resistant state.  |  Cerezo-Wallis, D., et al. 2020. Nat Med. 26: 1865-1877. PMID: 33077955
5. Midkine-A novel player in cardiovascular diseases.  |  Majaj, M. and Weckbach, LT. 2022. Front Cardiovasc Med. 9: 1003104. PMID: 36204583
6. Midkine promotes renal fibrosis by stabilizing C/EBPβ to facilitate endothelial-mesenchymal transition.  |  Xu, C., et al. 2024. Commun Biol. 7: 544. PMID: 38714800
7. Midkine as a driver of age-related changes and increase in mammary tumorigenesis.  |  Yan, P., et al. 2024. Cancer Cell. 42: 1936-1954.e9. PMID: 39366375
8. Midkine is a heat and acid stable polypeptide capable of enhancing plasminogen activator activity and neurite outgrowth extension.  |  Kojima, S., et al. 1995. Biochem Biophys Res Commun. 216: 574-81. PMID: 7488150
9. Increased midkine gene expression in human gastrointestinal cancers.  |  Aridome, K., et al. 1995. Jpn J Cancer Res. 86: 655-61. PMID: 7559083
10. A retinoic acid-responsive element in human midkine gene.  |  Pedraza, C., et al. 1995. J Biochem. 117: 845-9. PMID: 7592548
11. Midkine enhances fibrinolytic activity of bovine endothelial cells.  |  Kojima, S., et al. 1995. J Biol Chem. 270: 9590-6. PMID: 7721890
12. Localization of heparin-binding, neurite outgrowth and antigenic regions in midkine molecule.  |  Muramatsu, H., et al. 1994. Biochem Biophys Res Commun. 203: 1131-9. PMID: 8093031

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MK Antibody (A-9) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-MK Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MK Antibody (A-9) Specifications

Alternate Product Name: Midkine

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 79-143

Epitope Target: MK

Epitope Species: human

Product Reactivity/Detection: precursor and mature MK

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for MK Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin