MIP-3α CRISPR/Cas9 KO Plasmid (m): sc-422839
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MIP-3α CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MIP-3α genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MIP-3α CRISPR/Cas9 KO Plasmid (m) | sc-422839 | 20 µg | $397.00 |
Overview
Ccl20 encodes the chemokine macrophage inflammatory protein-3 alpha (MIP-3α), a secreted ligand for CCR6 that directs chemotaxis of immature dendritic cells, memory T cells, and B cells to sites of epithelial activation. Induced by NF-κB and proinflammatory cytokines, CCL20 participates in leukocyte trafficking, mucosal immune surveillance, and organization of inflammatory microenvironments. Dysregulated CCL20–CCR6 signaling has been associated with chronic inflammatory processes and autoimmune pathobiology, and it is frequently studied in the context of epithelial barrier dysfunction and tumor-associated immune cell recruitment. In mice, Ccl20 provides a tractable node for dissecting chemokine-driven cell migration and cytokine networks in immunology and inflammation research.
MIP-3α CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ccl20 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ccl20 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ccl20 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MIP-3α protein expression.
This CRISPR knockout system enables efficient generation of Ccl20-deficient cell models for investigation of MIP-3α signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ccl20 exon(s) critical for MIP-3α function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ccl20 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MIP-3α CRISPR/Cas9 KO Plasmid (m) and MIP-3α CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ccl20 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MIP-3α HDR Plasmid (m) and MIP-3α HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ccl20 homology arms to support homology-directed repair at defined Ccl20 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.