Mig-6 Antibody (D-1): sc-137154

Mig-6 Antibody (D-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Mig-6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Mig-6 Antibody (D-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The mitogen-inducible gene 6 protein (Mig-6), also known as Gene 33 or RALT, is a crucial cytoplasmic protein that plays a significant role as a feedback inhibitor of the ErbB-2 mitogenic pathway, thereby suppressing oncogenic activity. Mig-6 is vital for regulating cellular responses to growth factors, as Mig-6 binds to the epidermal growth factor receptor (EGFR) upon stimulation, acting as a negative feedback regulator and potentially functioning as a tumor suppressor. Notably, Mig-6 is involved in the transcriptional activation of NFκB by interacting with inhibitor IκB-α, which is essential for enabling cells to adapt to chronic stress conditions. Mig-6 expression is notably upregulated during cell growth and in response to various stressors, such as diabetic nephropathy and mechanical strain, highlighting Mig-6′s importance in cellular homeostasis. Furthermore, Mig-6 is predominantly expressed in the liver, placenta, and lung, underscoring Mig-6′s diverse physiological roles across different tissues.

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Mig-6 Antibody (D-1) References:

1. Gene 33/Mig-6, a transcriptionally inducible adapter protein that binds GTP-Cdc42 and activates SAPK/JNK. A potential marker transcript for chronic pathologic conditions, such as diabetic nephropathy. Possible role in the response to persistent stress.  |  Makkinje, A., et al. 2000. J Biol Chem. 275: 17838-47. PMID: 10749885
2. Inhibition of ErbB-2 mitogenic and transforming activity by RALT, a mitogen-induced signal transducer which binds to the ErbB-2 kinase domain.  |  Fiorentino, L., et al. 2000. Mol Cell Biol. 20: 7735-50. PMID: 11003669
3. Mig-6 is a negative regulator of the epidermal growth factor receptor signal.  |  Hackel, PO., et al. 2001. Biol Chem. 382: 1649-62. PMID: 11843178
4. A novel mechanism of nuclear factor kappaB activation through the binding between inhibitor of nuclear factor-kappaBalpha and the processed NH(2)-terminal region of Mig-6.  |  Tsunoda, T., et al. 2002. Cancer Res. 62: 5668-71. PMID: 12384522
5. Mitogen-inducible gene 6 (MIG-6), adipophilin and tuftelin are inducible by hypoxia.  |  Saarikoski, ST., et al. 2002. FEBS Lett. 530: 186-90. PMID: 12387890
6. Regulation of Gene33 expression by insulin requires MEK-ERK activation.  |  Keeton, AB., et al. 2004. Biochim Biophys Acta. 1679: 248-55. PMID: 15358516
7. Gene 33 is an endogenous inhibitor of epidermal growth factor (EGF) receptor signaling and mediates dexamethasone-induced suppression of EGF function.  |  Xu, D., et al. 2005. J Biol Chem. 280: 2924-33. PMID: 15556944
8. Mapping of the critical region of mitogene-inducible gene-6 for NF-kappaB activation.  |  Mabuchi, R., et al. 2005. Oncol Rep. 13: 473-6. PMID: 15706420
9. Mig-6 participates in the regulation of cell senescence and retinoblastoma protein phosphorylation.  |  Milewska, M. and Kolch, W. 2014. Cell Signal. 26: 1870-7. PMID: 24815188
10. Decreased Mitogen Inducible Gene 6 (MIG-6) Associated with Symptom Severity in Children with Autism.  |  Russo, A. 2014. Biomark Insights. 9: 85-9. PMID: 25342879
11. Loss of MIG-6 results in endometrial progesterone resistance via ERBB2.  |  Yoo, JY., et al. 2022. Nat Commun. 13: 1101. PMID: 35232969
12. Identification of a novel mitogen-inducible gene (mig-6): regulation during G1 progression and differentiation.  |  Wick, M., et al. 1995. Exp Cell Res. 219: 527-35. PMID: 7641805

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mig-6 Antibody (D-1) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-Mig-6 Antibody (D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mig-6 Antibody (D-1) Specifications

Alternate Product Name: Mig-6

Product Clone Name: D-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 156-280

Epitope Target: Mig-6

Epitope Species: human

Product Reactivity/Detection: Mig-6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for Mig-6 Antibody (D-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin