Mi2-β Antibody (F-7): sc-365639

Mi2-β Antibody (F-7) is a mouse monoclonal IgG3 kappa light chain antibody that detects Mi2-beta of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Mi2-beta antibody (F-7) is available as a non-conjugated format. Mi2-beta plays a crucial role in chromatin remodeling, which is essential for the regulation of gene expression. Mi2-beta (F-7) antibody is part of the mSin3 corepressor complex, which is involved in the deacetylation of histones, leading to transcriptional repression. This complex, which also includes histone deacetylases HDAC1 and HDAC2, is vital for maintaining the proper structure of chromatin and ensuring that genes are expressed at the right times and in the right amounts. The interaction of Mi2-beta with other proteins in this complex highlights its importance in cellular processes such as differentiation and development. Using Mi2-beta (F-7) antibody in various applications helps researchers gain insights into the mechanisms of transcriptional regulation and the role of chromatin dynamics in health and disease.

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Mi2-β Antibody (F-7) References:

1. The chromatin remodeler Mi-2beta is required for CD4 expression and T cell development.  |  Williams, CJ., et al. 2004. Immunity. 20: 719-33. PMID: 15189737
2. Antagonistic interactions between Ikaros and the chromatin remodeler Mi-2beta determine silencer activity and Cd4 gene expression.  |  Naito, T., et al. 2007. Immunity. 27: 723-34. PMID: 17980631
3. The role of the chromatin remodeler Mi-2beta in hematopoietic stem cell self-renewal and multilineage differentiation.  |  Yoshida, T., et al. 2008. Genes Dev. 22: 1174-89. PMID: 18451107
4. Mi2beta shows chromatin enzyme specificity by erasing a DNase I-hypersensitive site established by ACF.  |  Ishii, H., et al. 2009. J Biol Chem. 284: 7533-41. PMID: 19158090
5. The major dermatomyositis-specific Mi-2 autoantigen is a presumed helicase involved in transcriptional activation.  |  Seelig, HP., et al. 1995. Arthritis Rheum. 38: 1389-99. PMID: 7575689
6. Nucleosome structural changes due to acetylation.  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
7. A positive role for histone acetylation in transcription factor access to nucleosomal DNA.  |  Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
8. Transcriptional silencing in yeast is associated with reduced nucleosome acetylation.  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
9. Repression and activation by multiprotein complexes that alter chromatin structure.  |  Kingston, RE., et al. 1996. Genes Dev. 10: 905-20. PMID: 8608939
10. Histone deacetylases and SAP18, a novel polypeptide, are components of a human Sin3 complex.  |  Zhang, Y., et al. 1997. Cell. 89: 357-64. PMID: 9150135
11. SAP30, a novel protein conserved between human and yeast, is a component of a histone deacetylase complex.  |  Zhang, Y., et al. 1998. Mol Cell. 1: 1021-31. PMID: 9651585
12. The dermatomyositis-specific autoantigen Mi2 is a component of a complex containing histone deacetylase and nucleosome remodeling activities.  |  Zhang, Y., et al. 1998. Cell. 95: 279-89. PMID: 9790534

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Mi2-β Antibody (F-7) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Mi2-β Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Mi2-β Antibody (F-7) Specifications

Alternate Product Name: Mi2-β

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 1887-1909

Epitope Target: Mi2-β

Epitope Species: human

Product Reactivity/Detection: Mi2-β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Mi2-β Antibody (F-7): P: Blocking Peptide

The preferred Mi2 antibody is Mi2 Antibody (B-4): sc-55606.