MHC class IIβ Antibody (SW73.2): sc-101617

MHC class IIβ Antibody (SW73.2) is a rat monoclonal IgG2a antibody that detects MHC class II beta protein of ovine and caprine origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-MHC class II beta antibody (SW73.2) is available as a non-conjugated format. The major histocompatibility complex (MHC) molecules, also known as sheep leukocyte antigen (OLA) molecules, play a crucial role in the immune system by presenting foreign peptides to T lymphocytes, thereby initiating an immune response. MHC class II molecules are particularly important for the activation of helper T lymphocytes, which are essential for orchestrating the adaptive immune response. Structurally, MHC class II molecules consist of a non-covalent complex of an alpha and beta chain, allowing MHC class II molecules to bind and present 13-18 amino acid long antigenic peptides. The interaction between MHC class II molecules and T lymphocytes is vital for the recognition of pathogens and the subsequent activation of immune cells. Additionally, the presence of regulatory molecules such as HLA-DM and HLA-DO, which are found in endosomal and lysosomal compartments, ensures that the conformation of MHC class II molecules is optimal for peptide exchange, further enhancing the immune response. The distribution of MHC class IIβ in sheep tissues mirrors that found in humans, highlighting MHC class IIβ significance in comparative immunology and potential applications in veterinary medicine and research.

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MHC class IIβ Antibody (SW73.2) References:

1. Ovar-Mhc--ovine major histocompatibility complex: role in genetic resistance to diseases.  |  Dukkipati, VS., et al. 2006. N Z Vet J. 54: 153-60. PMID: 16915336
2. Distribution and origin of bovine major histocompatibility complex class II DQA1 genes in Japan.  |  Takeshima, S., et al. 2008. Tissue Antigens. 72: 195-205. PMID: 18715338
3. Differential expression of inflammatory and immune response genes in rams experimentally infected with a rough virulent strain of Brucella ovis.  |  Galindo, RC., et al. 2009. Vet Immunol Immunopathol. 127: 295-303. PMID: 19056128
4. Monoclonal antibodies to sheep lymphocytes. I. Identification of MHC class II molecules on lymphoid tissue and changes in the level of class II expression on lymph-borne cells following antigen stimulation in vivo.  |  Hopkins, J., et al. 1986. Immunology. 59: 433-8. PMID: 2431999
5. A novel MHC class II epitope expressed in thymic medulla but not cortex.  |  Murphy, DB., et al. 1989. Nature. 338: 765-8. PMID: 2469959
6. Monoclonal antibodies to sheep MHC class II molecules recognize all HLA-D or subsets of HLA-D region products.  |  Puri, NK., et al. 1987. Hum Immunol. 20: 195-207. PMID: 3501780
7. Sheep lymphocyte antigens (OLA). II. Major histocompatibility complex class II molecules.  |  Puri, NK., et al. 1985. Immunology. 56: 725-33. PMID: 3908294