MGST2 CRISPR/Cas9 KO Plasmid (m): sc-431759
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MGST2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MGST2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MGST2 CRISPR/Cas9 KO Plasmid (m) | sc-431759 | 20 µg | $397.00 |
Overview
Microsomal glutathione S-transferase 2 (MGST2), encoded by the mouse Mgst2 gene, is a membrane-associated enzyme that conjugates glutathione to reactive lipid intermediates and supports cellular detoxification under oxidative and inflammatory stress. MGST2 participates in eicosanoid metabolism, including leukotriene C4 biosynthesis, linking it to arachidonic acid–driven signaling pathways that regulate vascular tone, platelet function, and immune cell activation. By shaping redox homeostasis and lipid mediator production, MGST2 can influence mitochondrial and ER stress responses, membrane integrity, and pro-inflammatory transcriptional programs. Dysregulated glutathione-dependent detoxification and leukotriene signaling are relevant to models of asthma-like inflammation, atherosclerosis, and neuroinflammation, making Mgst2 a useful target for mechanistic studies of inflammatory lipid networks.
MGST2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mgst2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mgst2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mgst2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MGST2 protein expression.
This CRISPR knockout system enables efficient generation of Mgst2-deficient cell models for investigation of MGST2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mgst2 exon(s) critical for MGST2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mgst2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MGST2 CRISPR/Cas9 KO Plasmid (m) and MGST2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mgst2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MGST2 HDR Plasmid (m) and MGST2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mgst2 homology arms to support homology-directed repair at defined Mgst2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.