MGRN1 CRISPR/Cas9 KO Plasmid (m): sc-421607
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MGRN1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MGRN1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MGRN1 CRISPR/Cas9 KO Plasmid (m) | sc-421607 | 20 µg | $397.00 |
Overview
Mgrn1 encodes the E3 ubiquitin-protein ligase MGRN1, a regulator of ubiquitin-dependent protein turnover and endosomal–lysosomal trafficking in neurons and other cell types. MGRN1 modulates membrane protein sorting and receptor homeostasis, linking ubiquitination to vesicular transport and proteostasis pathways. In mouse, altered Mgrn1 function has been associated with neurodegeneration phenotypes and pigmentation abnormalities, supporting its relevance to studies of neuronal maintenance and melanosome biology. Dissecting MGRN1-dependent ubiquitin signaling is useful for understanding how defects in protein quality control and trafficking contribute to cellular stress responses.
MGRN1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mgrn1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mgrn1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mgrn1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MGRN1 protein expression.
This CRISPR knockout system enables efficient generation of Mgrn1-deficient cell models for investigation of MGRN1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mgrn1 exon(s) critical for MGRN1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mgrn1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MGRN1 CRISPR/Cas9 KO Plasmid (m) and MGRN1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mgrn1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MGRN1 HDR Plasmid (m) and MGRN1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mgrn1 homology arms to support homology-directed repair at defined Mgrn1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.