MGP Antibody (A-11): sc-271906

MGP Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Matrix Gla Protein (MGP) of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-MGP antibody (A-11) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Matrix Gla Protein plays a crucial role in regulating calcification within the extracellular matrix, particularly in cartilage and arterial tissues, which is vital for maintaining vascular health and preventing pathological calcification. MGP is a vitamin K-dependent protein characterized by the presence of five to six residues of γ-carboxy-glutamic acid (Gla), an amino acid that binds calcium ions and is essential for MGP′s function. In humans, MGP consists of 84 amino acids, along with a 19-amino-acid transmembrane signal peptide, while a truncated 77-residue form is found in human bone extracts, likely resulting from enzymatic processing. High expression levels of MGP are observed in the heart, kidney, and lung, with notable over-expression in the breast cancer cell line 600 PEI. Additionally, retinoic acid has been shown to induce MGP expression in chondrocytes, fibroblasts, and osteoblasts. Mutations in the MGP gene can lead to Keutel syndrome, a condition associated with abnormal cartilage calcification, highlighting MGP′s significant role in extracellular matrix calcification regulation. MGP can interact with vitronectin and fibronectin through its C-terminus, and phosphorylation occurs near the N-terminus at three serine residues, which are part of a tandemly repeated Ser-X-Glu sequence, further influencing biological activity.

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MGP Antibody (A-11) References:

1. Matrix Gla protein C-terminal region binds to vitronectin. Co-localization suggests binding occurs during tissue development.  |  Nishimoto, SK. and Nishimoto, M. 2005. Matrix Biol. 24: 353-61. PMID: 15982861
2. Matrix GLA protein gene polymorphisms: clinical correlates and cardiovascular mortality in chronic kidney disease patients.  |  Brancaccio, D., et al. 2005. Am J Nephrol. 25: 548-52. PMID: 16210837
3. Carboxyl-terminal proteolytic processing of matrix Gla protein.  |  Hale, JE., et al. 1991. J Biol Chem. 266: 21145-9. PMID: 1939157
4. Overexpression of matrix Gla protein mRNA in malignant human breast cells: isolation by differential cDNA hybridization.  |  Chen, L., et al. 1990. Oncogene. 5: 1391-5. PMID: 2216462
5. Molecular structure, chromosome assignment, and promoter organization of the human matrix Gla protein gene.  |  Cancela, L., et al. 1990. J Biol Chem. 265: 15040-8. PMID: 2394711
6. Matrix Gla protein, a new gamma-carboxyglutamic acid-containing protein which is associated with the organic matrix of bone.  |  Price, PA., et al. 1983. Biochem Biophys Res Commun. 117: 765-71. PMID: 6607731
7. Conserved phosphorylation of serines in the Ser-X-Glu/Ser(P) sequences of the vitamin K-dependent matrix Gla protein from shark, lamb, rat, cow, and human.  |  Price, PA., et al. 1994. Protein Sci. 3: 822-30. PMID: 8061611
8. Mutations in the gene encoding the human matrix Gla protein cause Keutel syndrome.  |  Munroe, PB., et al. 1999. Nat Genet. 21: 142-4. PMID: 9916809

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MGP Antibody (A-11) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-MGP Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MGP Antibody (A-11) Specifications

Alternate Product Name: Matrix Gla Protein

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-103

Epitope Target: MGP

Epitope Species: human

Product Reactivity/Detection: MGP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for MGP Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin