MGMT Antibody (SPM287): sc-56432

MGMT Antibody (SPM287) is a mouse monoclonal IgG1 kappa light chain antibody raised against purified recombinant MGMT protein of human origin, capable of detecting MGMT from mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). MGMT, also known as O6-methylguanine-DNA methyltransferase or O-6-alkylguanine DNA alkyltransferase (AGT), is a critical DNA repair enzyme that maintains genomic stability by removing mutagenic and cytotoxic alkyl groups from the O6 position of guanine, thus preventing mispairing during DNA replication. Structurally, MGMT is a single-domain protein consisting of approximately 207 amino acids, featuring a highly conserved active site with a cysteine residue (Cys145 in humans) that directly accepts the alkyl group from damaged DNA. The importance of MGMT′s structure lies in its unique mechanism of action, which has significant implications for cancer therapeutics, as inhibiting MGMT can enhance the efficacy of alkylating chemotherapeutic agents by preventing the repair of induced DNA damage in cancer cells. MGMT expression is transcriptionally activated in response to DNA damage, however, this induction does not occur in p53-deficient mice, suggesting that functional p53 is necessary for MGMT regulation. Studies have shown that MGMT mRNA and protein levels increase upon exposure to ionizing radiation only in cell lines expressing wild-type p53, indicating a regulatory relationship between MGMT and p53. Conversely, high MGMT activity has been associated with the presence of mutant p53 in certain cancer cell lines, such as those derived from oral cancers, while lower MGMT activity is observed in ovarian tumors with wild-type p53 compared to those with mutant p53, supporting the notion that wild-type p53 may down-regulate basal MGMT promoter activity. Understanding the structure and function of MGMT is essential in cancer research and therapeutic development, making anti-MGMT antibody (SPM287) a valuable tool for researchers investigating DNA repair mechanisms, protein structure-function relationships, and p53-related regulatory pathways.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MGMT Antibody (SPM287) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-MGMT Antibody (SPM287) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MGMT Antibody (SPM287) Specifications

Alternate Product Name: MGMT

Product Clone Name: SPM287

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: MGMT

Epitope Species: human

Product Reactivity/Detection: MGMT

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for MGMT Antibody (SPM287): N/A

The preferred MGMT antibody is MGMT Antibody (MT 3.1): sc-56157.