MGMT Antibody (G-10): sc-514277
- MGMT Antibody (G-10) is a mouse monoclonal IgG2b provided at 200 µg/ml
- specific for an epitope mapping between amino acids 185-207 at the C-terminus of MGMT of human origin
- recommended for detection of MGMT of human origin by WB, IP, IF and ELISA
- See MGMT (E-1): sc-166528 for MGMT antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for MGMT Antibody (G-10) for WB applications. These reagents are now offered in bundles with MGMT Antibody (G-10) (see ordering information below).
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MGMT Antibody (G-10) is a mouse monoclonal IgG2b antibody specifically designed to recognize an epitope mapping between amino acids 185-207 at the C-terminus of the human MGMT protein, also known as O6-methylguanine-DNA methyltransferase or O-6-alkylguanine DNA alkyltransferase (AGT). Anti-MGMT antibody (G-10) is recommended for use in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MGMT is a crucial DNA repair enzyme located primarily in the cell nucleus, where MGMT functions to remove mutagenic and cytotoxic alkyl groups from the O6 position of guanine, thereby preventing DNA replication errors and maintaining genomic stability. Structurally, MGMT consists of a single polypeptide chain with a conserved active site that facilitates the direct reversal of DNA alkylation damage, a process essential for protecting cells from carcinogenic agents. Post-translational modifications of MGMT, including ubiquitination, play a significant role in regulating stability and degradation, thereby controlling cellular levels and the efficiency of the DNA repair process. MGMT interacts with several key proteins, notably the tumor suppressor p53, which regulates expression in response to DNA damage. Functional p53 enhances MGMT transcription, while mutant p53 can disrupt this regulation, leading to altered MGMT activity. Additionally, MGMT′s interaction with ubiquitin ligases influences proteasomal degradation, highlighting the importance of these modifications in modulating activity and cellular response to DNA damage. Understanding these interactions and modifications is vital for cancer research, as elevated MGMT levels can confer resistance to alkylating chemotherapeutic agents, impacting treatment outcomes. MGMT monoclonal antibody (G-10) serves as an invaluable tool for researchers studying the intricate mechanisms of DNA repair, protein interactions, and the regulatory pathways involving MGMT, thereby contributing to advancements in cancer therapeutics and molecular biology.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MGMT Antibody (G-10) | sc-514277 | 200 µg/ml | $322.00 | |||
MGMT Antibody (G-10): m-IgG Fc BP-HRP Bundle | sc-540969 | 200 µg Ab; 10 µg BP | $361.00 | |||
MGMT Antibody (G-10): m-IgG2b BP-HRP Bundle | sc-549795 | 200 µg Ab; 10 µg BP | $361.00 | |||
MGMT (G-10) Neutralizing Peptide | sc-514277 P | 100 µg/0.5 ml | $69.00 |