mGluR-8a/b/c CRISPR/Cas9 KO Plasmid (m): sc-420695
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- mGluR-8a/b/c CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the mGluR-8a/b/c genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: mGluR-8 Antibody (4A7): sc-517124
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
mGluR-8a/b/c CRISPR/Cas9 KO Plasmid (m) | sc-420695 | 20 µg | $397.00 |
Overview
Grm8 encodes metabotropic glutamate receptor 8 (mGluR-8a/b/c), a group III GPCR that couples primarily to Gi/o proteins to reduce adenylyl cyclase activity, modulate cAMP/PKA signaling, and regulate presynaptic neurotransmitter release. mGluR8 activity influences synaptic transmission and plasticity by tuning ion channel function and vesicle release probability, integrating glutamatergic signaling across neural circuits. In mouse, Grm8 is studied in the context of excitatory/inhibitory balance and circuit-level mechanisms that shape sensory processing, learning, and stress-responsive behaviors. Dysregulated glutamate receptor signaling, including group III mGluRs, is frequently investigated for its contributions to neurodevelopmental and neuropsychiatric phenotypes in experimental models.
mGluR-8a/b/c CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Grm8 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Grm8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Grm8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish mGluR-8a/b/c protein expression.
This CRISPR knockout system enables efficient generation of Grm8-deficient cell models for investigation of mGluR-8a/b/c signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Grm8 exon(s) critical for mGluR-8a/b/c function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Grm8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by mGluR-8a/b/c CRISPR/Cas9 KO Plasmid (m) and mGluR-8a/b/c CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Grm8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by mGluR-8a/b/c HDR Plasmid (m) and mGluR-8a/b/c HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Grm8 homology arms to support homology-directed repair at defined Grm8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.