MGC29506 CRISPR/Cas9 KO Plasmid (h): sc-406694
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MGC29506 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MGC29506 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MGC29506 CRISPR/Cas9 KO Plasmid (h) | sc-406694 | 20 µg | $397.00 |
Overview
MZB1 (also known as MGC29506) encodes a lumenal endoplasmic reticulum protein enriched in B-lineage cells that supports plasma cell differentiation and immunoglobulin biosynthesis. It functions as a co-chaperone within the ER proteostasis network, coordinating oxidative folding and quality control through interactions with the GRP94/HSP90B1 and BiP/HSPA5 chaperone systems and contributing to ER stress and unfolded protein response signaling. By modulating secretory pathway capacity and handling of misfolded proteins, MZB1 influences antibody secretion programs and B-cell homeostasis. Dysregulated expression has been associated with aberrant plasma cell states and immune-related pathophysiology, making it relevant for studies of B-cell maturation, ER stress adaptation, and secretory cell biology.
MGC29506 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MZB1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MZB1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MZB1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MGC29506 protein expression.
This CRISPR knockout system enables efficient generation of MZB1-deficient cell models for investigation of MGC29506 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MZB1 exon(s) critical for MGC29506 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MZB1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MGC29506 CRISPR/Cas9 KO Plasmid (h) and MGC29506 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MZB1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MGC29506 HDR Plasmid (h) and MGC29506 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MZB1 homology arms to support homology-directed repair at defined MZB1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.