MGAT1 Double Nickase Plasmid (m): sc-427040-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MGAT1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MGAT1 Double Nickase Plasmid (m) and MGAT1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Mogat1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MGAT1 Antibody (H-6): sc-376079
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MGAT1 Double Nickase Plasmid (m) | sc-427040-NIC | 20 µg | $410.00 | |||
MGAT1 Double Nickase Plasmid (m2) | sc-427040-NIC-2 | 20 µg | $410.00 |
Mouse Mogat1 encodes MGAT1 (monoacylglycerol O-acyltransferase 1), an endoplasmic reticulum–associated enzyme that catalyzes acyl-CoA–dependent conversion of monoacylglycerol to diacylglycerol, providing substrate for triacylglycerol synthesis. By regulating diacylglycerol availability, MGAT1 links lipid storage programs with signaling processes influenced by DAG, intersecting with pathways that shape membrane lipid composition and metabolic stress responses. Mogat1 expression is prominent in metabolic tissues and is frequently studied in contexts of nutrient excess, hepatic lipid accumulation, and altered insulin sensitivity, where shifts in neutral lipid synthesis can remodel cellular homeostasis. These features make MGAT1 a relevant target for mechanistic studies of lipid metabolism, energy balance, and downstream transcriptional adaptations in mouse models.
MGAT1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Mogat1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Mogat1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Mogat1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Mogat1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.