MFG-E8 Antibody (H-3): sc-377356
- MFG-E8 Antibody (H-3) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 56-190 mapping near the N-terminus of MFG-E8 of mouse origin
- MFG-E8 Antibody (H-3) is recommended for detection of MFG-E8 of mouse origin by WB, IP, IF and ELISA
- Anti-MFG-E8 Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MFG-E8 Antibody (H-3). This work is in progress.
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SEE ALSO...
MFG-E8 Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects MFG-E8 of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MFG-E8 Antibody (H-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MFG-E8, also known as Milk fat globule-EGF factor 8 or Lactadherin, is a 387 amino acid peripheral membrane protein that plays a crucial role in cell adhesion and signaling. MFG-E8 is primarily located on mammary epithelial cell membranes and is abundant in human breast milk, where MFG-E8 encapsulates lipids and serves as a marker for differentiated carcinomas. MFG-E8 contains one epidermal growth factor-like domain and two F5/8 type C domains, which facilitate interaction with integrins, specifically Integrin β5 and Integrin β3. This interaction is vital for processes such as gamete binding and cell attachment, highlighting MFG-E8′s potential role in fertilization and apoptosis. MFG-E8 has been shown to bind to rotavirus, inhibiting viral replication and providing a protective effect against infections. Notably, overexpression of MFG-E8 has been linked to breast cancer, suggesting involvement in tumorigenesis and making MFG-E8 a significant target for cancer research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
MFG-E8 Antibody (H-3) References:
- Mesenchymal stem cells-derived MFG-E8 accelerates diabetic cutaneous wound healing. | Uchiyama, A., et al. 2017. J Dermatol Sci. 86: 187-197. PMID: 28302404
- Prognostic Correlation Between MFG-E8 Expression Level and Colorectal Cancer. | Jia, M., et al. 2017. Arch Med Res. 48: 270-275. PMID: 28923329
- The significance of tumor cells-derived MFG-E8 in tumor growth of angiosarcoma. | Fujiwara, C., et al. 2019. J Dermatol Sci. 96: 18-25. PMID: 31447183
- The expression levels and clinical significance of MFG-E8 and CD133 in epithelial ovarian cancer. | Li, N., et al. 2020. Gynecol Endocrinol. 36: 803-807. PMID: 31899997
- Propofol suppresses microglial phagocytosis through the downregulation of MFG-E8. | Cai, X., et al. 2021. J Neuroinflammation. 18: 18. PMID: 33422097
- MFG-E8 Regulates Vascular Smooth Muscle Cell Migration Through Dose-Dependent Mediation of Actin Polymerization. | Chiang, HY., et al. 2021. J Am Heart Assoc. 10: e020870. PMID: 34041925
- CX3CL1(+) Microparticles-Induced MFG-E8 Enhances Apoptotic Cell Clearance by Alveolar Macrophages. | Tsai, WH., et al. 2021. Cells. 10: PMID: 34685562
- MFG-E8 alleviates intervertebral disc degeneration by suppressing pyroptosis and extracellular matrix degradation in nucleus pulposus cells via Nrf2/TXNIP/NLRP3 axis. | Ma, H., et al. 2022. Cell Death Discov. 8: 209. PMID: 35440086
- Astrocyte-derived MFG-E8 facilitates microglial synapse elimination in Alzheimer's disease mouse models. | Sokolova, D., et al. 2024. bioRxiv.. PMID: 39257734
- MFGE8 promotes gastric cancer progression by activating the IL-6/JAK/STAT3 signaling. | Ding, LL., et al. 2025. Cell Signal. 125: 111486. PMID: 39490801
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MFG-E8 Antibody (H-3) | sc-377356 | 200 µg/ml | $322.00 | |||
MFG-E8 Antibody (H-3) AC | sc-377356 AC | 500 µg/ml, 25% agarose | $424.00 | |||
MFG-E8 Antibody (H-3) HRP | sc-377356 HRP | 200 µg/ml | $322.00 | |||
MFG-E8 Antibody (H-3) FITC | sc-377356 FITC | 200 µg/ml | $336.00 | |||
MFG-E8 Antibody (H-3) PE | sc-377356 PE | 200 µg/ml | $349.00 | |||
MFG-E8 Antibody (H-3) Alexa Fluor® 488 | sc-377356 AF488 | 200 µg/ml | $364.00 | |||
MFG-E8 Antibody (H-3) Alexa Fluor® 546 | sc-377356 AF546 | 200 µg/ml | $364.00 | |||
MFG-E8 Antibody (H-3) Alexa Fluor® 594 | sc-377356 AF594 | 200 µg/ml | $364.00 | |||
MFG-E8 Antibody (H-3) Alexa Fluor® 647 | sc-377356 AF647 | 200 µg/ml | $364.00 | |||
MFG-E8 Antibody (H-3) Alexa Fluor® 680 | sc-377356 AF680 | 200 µg/ml | $364.00 | |||
MFG-E8 Antibody (H-3) Alexa Fluor® 790 | sc-377356 AF790 | 200 µg/ml | $364.00 |