MFG-E8 Antibody (F-5): sc-271574

MFG-E8 Antibody (F-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects MFG-E8 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MFG-E8 Antibody (F-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MFG-E8, also known as Milk fat globule-EGF factor 8 or Lactadherin, is a 387 amino acid peripheral membrane protein that plays a crucial role in cellular interactions and signaling. MFG-E8 is primarily located on the membranes of mammary epithelial cells and other tissues, where MFG-E8 serves as a specific ligand for integrins β3 and β5, facilitating cell adhesion and communication. This interaction is particularly important in processes such as fertilization and apoptosis, as MFG-E8 helps regulate the attachment of cells to their extracellular matrix and influences cell survival. Furthermore, MFG-E8 has been shown to bind to rotavirus, inhibiting viral replication and providing a protective mechanism against infections. MFG-E8 overexpression has been linked to breast cancer, indicating potential involvement in tumorigenesis and making MFG-E8 a significant target for cancer research.

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MFG-E8 Antibody (F-5) References:

1. Correction of MFG-E8 Resolves Inflammation and Promotes Cutaneous Wound Healing in Diabetes.  |  Das, A., et al. 2016. J Immunol. 196: 5089-100. PMID: 27194784
2. MFG-E8 overexpression promotes colorectal cancer progression via AKT/MMPs signalling.  |  Zhao, Q., et al. 2017. Tumour Biol. 39: 1010428317707881. PMID: 28653875
3. Immunoregulatory influence of abundant MFG-E8 expression by esophageal cancer treated with chemotherapy.  |  Kanemura, T., et al. 2018. Cancer Sci. 109: 3393-3402. PMID: 30156356
4. The structure and properties of MFG-E8 and the In vitro assessment of its toxic effects on myoblast cells.  |  Li, H., et al. 2021. Protein Expr Purif. 178: 105720. PMID: 32771447
5. MFG-E8 regulated by miR-99b-5p protects against osteoarthritis by targeting chondrocyte senescence and macrophage reprogramming via the NF-κB pathway.  |  Lu, Y., et al. 2021. Cell Death Dis. 12: 533. PMID: 34031369
6. DCI after Aneurysmal Subarachnoid Hemorrhage Is Related to the Expression of MFG-E8.  |  Chen, X., et al. 2021. Biomed Res Int. 2021: 6568477. PMID: 35005020
7. αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8.  |  Mai, J., et al. 2022. BMC Biotechnol. 22: 15. PMID: 35597930
8. MFG-E8 Knockout Aggravated Nonalcoholic Steatohepatitis by Promoting the Activation of TLR4/NF-κB Signaling in Mice.  |  Hu, J., et al. 2022. Mediators Inflamm. 2022: 5791915. PMID: 35769208
9. Elevated MFG-E8 in CSF in the Early Stage Indicates Rapid Recovery of Mild Aneurysmal SAH Patients.  |  Pang, C., et al. 2022. Dis Markers. 2022: 6731286. PMID: 36267465
10. MFG-E8 Ameliorates Nerve Injury-Induced Neuropathic Pain by Regulating Microglial Polarization and Neuroinflammation via Integrin β3/SOCS3/STAT3 Pathway in Mice.  |  Zhang, L., et al. 2024. J Neuroimmune Pharmacol. 19: 49. PMID: 39305375

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MFG-E8 Antibody (F-5) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-MFG-E8 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MFG-E8 Antibody (F-5) Specifications

Alternate Product Name: Milk Fat Globule-EGF Factor 8

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 325-384

Epitope Target: MFG-E8

Epitope Species: human

Product Reactivity/Detection: MFG-E8

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for MFG-E8 Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MFG-E8 antibody is MFG-E8 Antibody (MFG-06): sc-8029.