METTL6 Double Nickase Plasmid (h): sc-416547-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- METTL6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- METTL6 Double Nickase Plasmid (h) and METTL6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting METTL6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: METTL6 Antibody (F-8): sc-515214
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
METTL6 Double Nickase Plasmid (h) | sc-416547-NIC | 20 µg | $410.00 | |||
METTL6 Double Nickase Plasmid (h2) | sc-416547-NIC-2 | 20 µg | $410.00 |
METTL6 encodes a S-adenosyl-L-methionine–dependent methyltransferase implicated in RNA modification, with reported activity toward specific cytosolic tRNAs that can influence translation fidelity and proteome output. Through shaping tRNA methylation patterns, METTL6 is linked to cellular processes such as translational control, proteostasis, and stress adaptation, which can secondarily affect proliferation and differentiation programs. Dysregulation of RNA modification enzymes, including tRNA methyltransferases, has been associated with altered growth phenotypes and transcriptome-to-proteome coupling in human disease contexts, making METTL6 a relevant target for mechanistic studies. In human cell models, perturbing METTL6 enables interrogation of how tRNA epitranscriptomic marks interface with gene expression regulation and cell-state transitions.
METTL6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the METTL6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within METTL6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt METTL6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of METTL6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.