METTL5 CRISPR/Cas9 KO Plasmid (h): sc-418556
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- METTL5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the METTL5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
METTL5 CRISPR/Cas9 KO Plasmid (h) | sc-418556 | 20 µg | $397.00 |
Overview
METTL5 encodes a SAM-dependent RNA methyltransferase that catalyzes N6-methyladenosine (m6A) deposition on 18S rRNA, helping shape ribosome structure and translational output. This modification is linked to ribosome biogenesis and fine-tuning of protein synthesis programs that support cellular growth and stress adaptation. Altered regulation of rRNA methylation machinery, including METTL5 activity, has been associated with changes in translational control observed in neurodevelopmental phenotypes and cancer-related gene expression states. As a result, METTL5 is studied in pathways connecting RNA epigenetics to proteostasis, cell-cycle progression, and differentiation.
METTL5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the METTL5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the METTL5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the METTL5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish METTL5 protein expression.
This CRISPR knockout system enables efficient generation of METTL5-deficient cell models for investigation of METTL5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting METTL5 exon(s) critical for METTL5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple METTL5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by METTL5 CRISPR/Cas9 KO Plasmid (h) and METTL5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the METTL5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by METTL5 HDR Plasmid (h) and METTL5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by METTL5 homology arms to support homology-directed repair at defined METTL5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.