METT10D CRISPR/Cas9 KO Plasmid (h): sc-408605
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- METT10D CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the METT10D genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
METT10D CRISPR/Cas9 KO Plasmid (h) | sc-408605 | 20 µg | $397.00 |
Overview
Human METTL16 (METT10D) encodes an S-adenosyl-L-methionine–dependent RNA methyltransferase that installs N6-methyladenosine (m6A) on structured RNA substrates, linking RNA modification to control of RNA metabolism. METTL16-mediated methylation can influence pre-mRNA splicing, RNA stability, and translation through effects on RNA structure and ribonucleoprotein assembly, and it has been connected to regulation of cellular methyl-donor homeostasis via MAT2A transcript processing. Through these functions, METTL16 participates in gene expression programs that shape cell growth and stress adaptation, making it relevant to studies of epitranscriptomic regulation in development and disease contexts. Altered METTL16 activity has been implicated in dysregulated RNA processing and proliferative signaling phenotypes observed across multiple cancer and neurobiology research models.
METT10D CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the METTL16 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the METTL16 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the METTL16 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish METT10D protein expression.
This CRISPR knockout system enables efficient generation of METTL16-deficient cell models for investigation of METT10D signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting METTL16 exon(s) critical for METT10D function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple METTL16 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by METT10D CRISPR/Cas9 KO Plasmid (h) and METT10D CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the METTL16 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by METT10D HDR Plasmid (h) and METT10D HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by METTL16 homology arms to support homology-directed repair at defined METTL16 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.