MetRS Double Nickase Plasmid (h): sc-402763-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MetRS Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MetRS Double Nickase Plasmid (h) and MetRS Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MARS. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MetRS Antibody (H-1): sc-166850
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MetRS Double Nickase Plasmid (h) | sc-402763-NIC | 20 µg | $410.00 | |||
MetRS Double Nickase Plasmid (h2) | sc-402763-NIC-2 | 20 µg | $410.00 |
Human MARS encodes methionyl‑tRNA synthetase (MetRS), a cytosolic aminoacyl‑tRNA synthetase that charges tRNA^Met with methionine to initiate and sustain protein synthesis. MetRS functions at the core of translational control and supports cellular proteostasis, linking nutrient availability and stress responses to global mRNA translation. As part of the multi‑aminoacyl‑tRNA synthetase complex, MetRS contributes to coordinated regulation of translation and signaling processes that influence proliferation and differentiation. Dysregulation of MARS expression or MetRS activity has been associated with altered translational capacity observed in cancer and neurodevelopmental and neurodegenerative disease contexts, making it relevant for mechanistic studies of proteome maintenance.
MetRS Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MARS locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MARS. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MARS function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MARS-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.