MetRS Antibody (H-1): sc-166850

MetRS Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects MetRS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-MetRS antibody (H-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MetRS, or methionyl-tRNA synthetase, plays a crucial role in protein synthesis by catalyzing the attachment of methionine to its corresponding tRNA, forming methionyl-tRNA. This process is vital for initiating translation, as methionine is the first amino acid incorporated into nascent polypeptide chains. MetRS′s ability to accurately recognize the anticodon and aminoacylation regions, which are spatially separated, underscores precision and efficiency in protein synthesis. Additionally, MetRS is characterized by an allosteric nature, where binding of trinucleotide anticodons occurs at a site distinct from the catalytic domains, allowing for finely tuned regulation of enzymatic activity. This intricate mechanism highlights MetRS′s importance in maintaining the fidelity of protein translation, making MetRS a significant target for research in cellular biology and therapeutic applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MetRS Antibody (H-1) References:

1. CHOP/GADD153 and methionyl-tRNA synthetase (MetRS) genes overlap in a conserved region that controls mRNA stability.  |  Ubeda, M., et al. 1999. Biochem Biophys Res Commun. 262: 31-8. PMID: 10448063
2. Nucleolar localization of human methionyl-tRNA synthetase and its role in ribosomal RNA synthesis.  |  Ko, YG., et al. 2000. J Cell Biol. 149: 567-74. PMID: 10791971
3. The appended C-domain of human methionyl-tRNA synthetase has a tRNA-sequestering function.  |  Kaminska, M., et al. 2001. Biochemistry. 40: 14309-16. PMID: 11714285
4. Protective mechanisms against homocysteine toxicity: the role of bleomycin hydrolase.  |  Zimny, J., et al. 2006. J Biol Chem. 281: 22485-92. PMID: 16769724
5. Impact of constitutive IGF1/IGF2 stimulation on the transcriptional program of human breast cancer cells.  |  Pacher, M., et al. 2007. Carcinogenesis. 28: 49-59. PMID: 16774935
6. Hierarchical network between the components of the multi-tRNA synthetase complex: implications for complex formation.  |  Han, JM., et al. 2006. J Biol Chem. 281: 38663-7. PMID: 17062567
7. Mutations in methylenetetrahydrofolate reductase or cystathionine beta-synthase gene, or a high-methionine diet, increase homocysteine thiolactone levels in humans and mice.  |  Chwatko, G., et al. 2007. FASEB J. 21: 1707-13. PMID: 17327360
8. Identification of novel inhibitors of methionyl-tRNA synthetase (MetRS) by virtual screening.  |  Finn, J., et al. 2008. Bioorg Med Chem Lett. 18: 3932-7. PMID: 18590962
9. Brucella melitensis Methionyl-tRNA-Synthetase (MetRS), a Potential Drug Target for Brucellosis.  |  Ojo, KK., et al. 2016. PLoS One. 11: e0160350. PMID: 27500735
10. A mutant methionyl-tRNA synthetase-based toolkit to assess induced-mesenchymal stromal cell secretome in mixed-culture disease models.  |  Burgess, JD., et al. 2023. Stem Cell Res Ther. 14: 289. PMID: 37798772
11. Methionyl-tRNA synthetase synthetic and proofreading activities are determinants of antibiotic persistence.  |  Wood, WN., et al. 2024. Front Microbiol. 15: 1384552. PMID: 38601944
12. Cloning of a human cDNA encoding a protein with high homology to yeast methionyl-tRNA synthetase.  |  Lage, H. and Dietel, M. 1996. Gene. 178: 187-9. PMID: 8921912

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MetRS Antibody (H-1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-MetRS Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MetRS Antibody (H-1) Specifications

Alternate Product Name: MetRS

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 121-420

Epitope Target: MetRS

Epitope Species: human

Product Reactivity/Detection: MetRS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for MetRS Antibody (H-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin