MetAP-1 Antibody (A-2): sc-514653

MetAP-1 Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects MetAP-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MetAP-1 (A-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Methionine aminopeptidases, or MetAPs, are crucial enzymes that play a significant role in protein maturation by removing the amino-terminal methionine residue from nascent polypeptides, a process essential for proper protein folding and function. MetAP-1 protein, consisting of 394 amino acids, is expressed at low levels across various tissues but shows particularly high expression in skeletal muscle, highlighting MetAP-1′s importance in muscle physiology. The active site of MetAP-1 is characterized by the presence of two adjacent divalent metal ions, which are essential for enzymatic activity, as these ions facilitate the hydrolysis of the methionine residue. This mechanism of action is evolutionarily conserved and underscores MetAP-1′s critical role in regulating cell proliferation in mammalian cells. Inhibitors such as pyridine-2-carboxylic acid thiazol-2-ylamide and 1,2,4-triazol have been identified, which target the active site of MetAP-1, providing insights into potential therapeutic strategies for diseases linked to dysregulated protein processing.

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MetAP-1 Antibody (A-2) References:

1. The 1.15A crystal structure of the Staphylococcus aureus methionyl-aminopeptidase and complexes with triazole based inhibitors.  |  Oefner, C., et al. 2003. J Mol Biol. 332: 13-21. PMID: 12946343
2. A single amino acid residue defines the difference in ovalicin sensitivity between type I and II methionine aminopeptidases.  |  Brdlik, CM. and Crews, CM. 2004. J Biol Chem. 279: 9475-80. PMID: 14676204
3. Inhibitors of type I MetAPs containing pyridine-2-carboxylic acid thiazol-2-ylamide. Part 1: SAR studies on the determination of the key scaffold.  |  Luo, QL., et al. 2005. Bioorg Med Chem Lett. 15: 635-8. PMID: 15664828
4. Inhibitors of type I MetAPs containing pyridine-2-carboxylic acid thiazol-2-ylamide. Part 2: SAR studies on the pyridine ring 3-substituent.  |  Luo, QL., et al. 2005. Bioorg Med Chem Lett. 15: 639-44. PMID: 15664829
5. Methionine aminopeptidases type I and type II are essential to control cell proliferation.  |  Bernier, SG., et al. 2005. J Cell Biochem. 95: 1191-203. PMID: 15962312
6. Molecular discrimination of type-I over type-II methionyl aminopeptidases.  |  Swierczek, K., et al. 2005. Biochemistry. 44: 12049-56. PMID: 16142902
7. Structural basis for the functional differences between type I and type II human methionine aminopeptidases.  |  Addlagatta, A., et al. 2005. Biochemistry. 44: 14741-9. PMID: 16274222
8. Structural basis of catalysis by monometalated methionine aminopeptidase.  |  Ye, QZ., et al. 2006. Proc Natl Acad Sci U S A. 103: 9470-5. PMID: 16769889

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MetAP-1 Antibody (A-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-MetAP-1 Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MetAP-1 Antibody (A-2) Specifications

Alternate Product Name: MetAP-1

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 67-89

Epitope Target: MetAP-1

Epitope Species: human

Product Reactivity/Detection: MetAP-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for MetAP-1 Antibody (A-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin