Metallothionein 3 CRISPR/Cas9 KO Plasmid (m): sc-421733
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Metallothionein 3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Metallothionein 3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Metallothionein 3 CRISPR/Cas9 KO Plasmid (m) | sc-421733 | 20 µg | $397.00 |
Overview
Mt3 encodes metallothionein 3 (MT3), a cysteine-rich metal-binding protein that regulates intracellular zinc and copper availability and contributes to cellular defense against oxidative and electrophilic stress. By buffering redox-active metals and shaping zinc-dependent signaling, MT3 can influence pathways linked to reactive oxygen species homeostasis, proteostasis, and mitochondrial function. In mouse tissues, MT3 expression is prominent in the nervous system, where it has been studied in relation to neuronal differentiation, synaptic function, and metal-associated neurotoxicity. Dysregulated metal handling and oxidative stress mechanisms involving MT3 are relevant to models of neurodegeneration and inflammation-associated tissue injury.
Metallothionein 3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mt3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mt3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mt3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Metallothionein 3 protein expression.
This CRISPR knockout system enables efficient generation of Mt3-deficient cell models for investigation of Metallothionein 3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mt3 exon(s) critical for Metallothionein 3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mt3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Metallothionein 3 CRISPR/Cas9 KO Plasmid (m) and Metallothionein 3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mt3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Metallothionein 3 HDR Plasmid (m) and Metallothionein 3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mt3 homology arms to support homology-directed repair at defined Mt3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.