Met Antibody (B-2): sc-8057

Met Antibody (B-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Met of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Met monoclonal antibody (B-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Met, also known as c-Met, is a transmembrane receptor with intrinsic tyrosine kinase activity, playing a crucial role in cellular processes such as proliferation, motility, and morphogenesis. Met function is particularly important in the context of tissue regeneration and repair, as Met serves as the receptor for hepatocyte growth factor, a key player in liver regeneration. Dysregulation of c-Met signaling has been implicated in various cancers, making Met a significant target for therapeutic intervention. The c-Met oncogene was first identified through transfection analysis in NIH/3T3 cells derived from a chemically induced human osteogenic sarcoma, highlighting Met relevance in oncogenesis. Furthermore, the presence of tyrosine phosphorylation of Met in certain human gastric carcinoma cell lines underscores Met potential role in tumor progression and metastasis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Met Antibody (B-2) References:

1. c-Met and hepatocyte growth factor: potential as novel targets in cancer therapy.  |  Sattler, M. and Salgia, R. 2007. Curr Oncol Rep. 9: 102-8. PMID: 17288874
2. In vivo detection of c-MET expression in a rat hepatocarcinogenesis model using molecularly targeted magnetic resonance imaging.  |  Towner, RA., et al. 2007. Mol Imaging. 6: 18-29. PMID: 17311762
3. Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product.  |  Bottaro, DP., et al. 1991. Science. 251: 802-4. PMID: 1846706
4. Mechanism of met oncogene activation.  |  Park, M., et al. 1986. Cell. 45: 895-904. PMID: 2423252
5. Characterization of the mouse met proto-oncogene.  |  Chan, AM., et al. 1988. Oncogene. 2: 593-9. PMID: 2838789
6. Amplification and overexpression of the met gene in spontaneously transformed NIH3T3 mouse fibroblasts.  |  Cooper, CS., et al. 1986. EMBO J. 5: 2623-8. PMID: 3023053
7. p145, a protein with associated tyrosine kinase activity in a human gastric carcinoma cell line.  |  Giordano, S., et al. 1988. Mol Cell Biol. 8: 3510-7. PMID: 3211149
8. Targeting MET Dysregulation in Cancer.  |  Recondo, G., et al. 2020. Cancer Discov. 10: 922-934. PMID: 32532746
9. The role of MET in chemotherapy resistance.  |  Wood, GE., et al. 2021. Oncogene. 40: 1927-1941. PMID: 33526881
10. MET-Activating Ubiquitin Multimers.  |  Kawakami, N., et al. 2023. Angew Chem Int Ed Engl. 62: e202307157. PMID: 37450419
11. Molecular cloning of a new transforming gene from a chemically transformed human cell line.  |  Cooper, CS., et al. Nature. 311: 29-33. PMID: 6590967
12. Phosphorylation of serine 985 negatively regulates the hepatocyte growth factor receptor kinase.  |  Gandino, L., et al. 1994. J Biol Chem. 269: 1815-20. PMID: 8294430

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Met Antibody (B-2) has 125 citations in a variety of scientific publications.

Primary antibodies like Anti-Met Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Met Antibody (B-2) Specifications

Alternate Product Name: c-Met

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1359-1379

Epitope Target: c-Met p140

Epitope Species: mouse

Product Reactivity/Detection: Met

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 125 citations in various scientific publications

Available Conjugates/Forms for Met Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin