Mel-CAM Antibody (2Q401): sc-71565

Mel-CAM Antibody (2Q401) is a mouse monoclonal IgG1 antibody that detects Mel-CAM in mouse, rat, human, canine, and rabbit samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Mel-CAM, also known as MCAM, MUC18, or CD146, is a transmembrane glycoprotein encoded by the human Mel-CAM gene, which plays a crucial role in cell adhesion and is a member of the immunoglobulin superfamily. This protein consists of 603 amino acids, featuring a signal peptide, five immunoglobulin-like domains, a transmembrane region, and a short cytoplasmic tail, which are essential for its function. Mel-CAM is predominantly expressed in advanced primary and metastatic melanomas, with its presence correlating strongly with the progression of metastatic disease, as Mel-CAM is found in 80% of advanced primary human melanomas but absent in normal melanocytes. Mel-CAM′s importance lies in its ability to initiate an outside-in signaling pathway involving protein tyrosine kinases such as Fyn, FAK, and paxillin, which subsequently influences the dynamics of actin cytoskeleton rearrangement. This function is vital not only for tumor progression but also for maintaining thymic architecture and function, highlighting Mel-CAM′s significance in both cancer biology and immune system integrity. Anti-Mel-CAM antibody (2Q401) is an invaluable tool for researchers investigating the mechanisms of melanoma progression and the role of cell adhesion in tumor biology.

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Mel-CAM Antibody (2Q401) References:

1. Expression of the cell adhesion molecule CD146/MCAM in non-small cell lung cancer.  |  Kristiansen, G., et al. 2003. Anal Cell Pathol. 25: 77-81. PMID: 12632016
2. MCAM is a novel metastasis marker and regulates spreading, apoptosis and invasion of ovarian cancer cells.  |  Wu, Z., et al. 2012. Tumour Biol. 33: 1619-28. PMID: 22610942
3. MCAM expression is associated with poor prognosis in non-small cell lung cancer.  |  Zhang, X., et al. 2014. Clin Transl Oncol. 16: 178-83. PMID: 23749325
4. ZBTB7A Suppresses Melanoma Metastasis by Transcriptionally Repressing MCAM.  |  Liu, XS., et al. 2015. Mol Cancer Res. 13: 1206-17. PMID: 25995384
5. The signalling receptor MCAM coordinates apical-basal polarity and planar cell polarity during morphogenesis.  |  Gao, Q., et al. 2017. Nat Commun. 8: 15279. PMID: 28589943
6. Critical role of the MCAM-ETV4 axis triggered by extracellular S100A8/A9 in breast cancer aggressiveness.  |  Chen, Y., et al. 2019. Neoplasia. 21: 627-640. PMID: 31100639
7. MCAM/CD146 Signaling via PLCγ1 Leads to Activation of β1-Integrins in Memory T-Cells Resulting in Increased Brain Infiltration.  |  Zondler, L., et al. 2020. Front Immunol. 11: 599936. PMID: 33381120
8. MCAM+CD161- Th17 Subset Expressing CD83 Enhances Tc17 Response in Psoriasis.  |  Maeda, K., et al. 2023. J Immunol. 210: 1867-1881. PMID: 37186262
9. Low fluid shear stress stimulates the uptake of noxious endothelial extracellular vesicles via MCAM and PECAM-1 cell adhesion molecules.  |  Coly, PM., et al. 2024. J Extracell Vesicles. 13: e12414. PMID: 39400522
10. Regulation of Mel-CAM/MUC18 expression on melanocytes of different stages of tumor progression by normal keratinocytes.  |  Shih, IM., et al. 1994. Am J Pathol. 145: 837-45. PMID: 7943174

The preferred Mel-CAM antibody is Mel-CAM Antibody (P1H12): sc-18837.