MEK kinase-1 Antibody (1-9C-2A): sc-449

MEK kinase-1 Antibody (1-9C-2A) is a mouse monoclonal IgG1 kappa light chain antibody that detects MEK kinase-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). MEK kinase-1 (1-9C-2A) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. MEK kinase-1 plays a crucial role in the mitogen-activated protein kinase (MAPK) signaling pathway, which is essential for cellular responses to growth factors and other extracellular signals. MEK kinase-1 is responsible for phosphorylating and activating MAP2K1 and MAP2K4, which in turn activate the extracellular signal-regulated kinases (ERK) and c-Jun NH(2)-terminal kinase (JNK) pathways. This activation is vital for regulating various cellular processes, including proliferation, differentiation, and survival. MEK kinase-1 is involved in the NFκB signaling pathway, activating central protein kinases such as CHUK and IKBKB, which are important for immune responses and inflammation. MEK kinase-1 utilizes an E3 ligase through its PHD domain for targeting proteins for degradation via ubiquitination, highlighting its role in maintaining cellular homeostasis and regulating protein levels within the cell.

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MEK kinase-1 Antibody (1-9C-2A) References:

1. Differential involvement of MEK kinase 1 (MEKK1) in the induction of apoptosis in response to microtubule-targeted drugs versus DNA damaging agents.  |  Gibson, S., et al. 1999. J Biol Chem. 274: 10916-22. PMID: 10196170
2. MEK kinase 1 induces mitochondrial permeability transition leading to apoptosis independent of cytochrome c release.  |  Gibson, EM., et al. 2002. J Biol Chem. 277: 10573-80. PMID: 11756439
3. A role for MEK kinase 1 in TGF-beta/activin-induced epithelium movement and embryonic eyelid closure.  |  Zhang, L., et al. 2003. EMBO J. 22: 4443-54. PMID: 12941696
4. Ablation of MEK kinase 1 suppresses intimal hyperplasia by impairing smooth muscle cell migration and urokinase plasminogen activator expression in a mouse blood-flow cessation model.  |  Li, Y., et al. 2005. Circulation. 111: 1672-8. PMID: 15795331
5. MEK kinase 1 is essential for Bcr-Abl-induced STAT3 and self-renewal activity in embryonic stem cells.  |  Nakamura, Y., et al. 2005. Oncogene. 24: 7592-8. PMID: 16044153
6. MEK kinase 1: kinase domain deficiency in mice reveals a role in orchestrating the thymus-dependent immunity and TNFR family signaling.  |  Gallagher, E. 2011. Adv Exp Med Biol. 691: 391-7. PMID: 21153343
7. A divergence in the MAP kinase regulatory network defined by MEK kinase and Raf.  |  Lange-Carter, CA., et al. 1993. Science. 260: 315-9. PMID: 8385802
8. Cloning of rat MEK kinase 1 cDNA reveals an endogenous membrane-associated 195-kDa protein with a large regulatory domain.  |  Xu, S., et al. 1996. Proc Natl Acad Sci U S A. 93: 5291-5. PMID: 8643568
9. Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase.  |  Wang, XS., et al. 1996. J Biol Chem. 271: 31607-11. PMID: 8940179
10. Cloning of a novel mitogen-activated protein kinase kinase kinase, MEKK4, that selectively regulates the c-Jun amino terminal kinase pathway.  |  Gerwins, P., et al. 1997. J Biol Chem. 272: 8288-95. PMID: 9079650
11. MEK kinases are regulated by EGF and selectively interact with Rac/Cdc42.  |  Fanger, GR., et al. 1997. EMBO J. 16: 4961-72. PMID: 9305638
12. Grb2 interaction with MEK-kinase 1 is involved in regulation of Jun-kinase activities in response to epidermal growth factor.  |  Pomérance, M., et al. 1998. J Biol Chem. 273: 24301-4. PMID: 9733714

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MEK kinase-1 Antibody (1-9C-2A) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-MEK kinase-1 Antibody (1-9C-2A) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MEK kinase-1 Antibody (1-9C-2A) Specifications

Alternate Product Name: MAP3K1

Product Clone Name: 1-9C-2A

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-301

Epitope Target: MEK kinase-1

Epitope Species: human

Product Reactivity/Detection: MEK kinase-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for MEK kinase-1 Antibody (1-9C-2A): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin