Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MEK-7 Double Nickase Plasmid (h): sc-402363-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MEK-7 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MEK-7 Double Nickase Plasmid (h) and MEK-7 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MAP2K7. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MEK-7 Antibody (E-7): sc-25288
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MEK-7 Double Nickase Plasmid (h) | sc-402363-NIC | 20 µg | $410.00 | |||
MEK-7 Double Nickase Plasmid (h2) | sc-402363-NIC-2 | 20 µg | $410.00 |
MAP2K7 encodes MEK-7, a dual-specificity MAP kinase kinase that preferentially phosphorylates and activates JNK isoforms, linking diverse extracellular stress and cytokine cues to transcriptional reprogramming. MEK-7 functions within the MAPK cascade alongside upstream MAP3Ks to regulate apoptosis, inflammatory signaling, and cytoskeletal remodeling through downstream targets such as c-JUN and ATF family transcription factors. Perturbation of MAP2K7-dependent JNK signaling has been associated with altered immune responses and oncogenic phenotypes in multiple tissue contexts, making it a useful node for dissecting stress-activated pathway control. Human MEK-7 is therefore widely studied in models of inflammation, neurobiology, and cancer cell signaling where pathway specificity and signaling dynamics are critical.
MEK-7 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MAP2K7 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MAP2K7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MAP2K7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MAP2K7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.