MEK-5 Antibody (E-3): sc-365198

MEK-5 Antibody (E-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects MEK-5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MEK-5 antibody (E-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MEK-5, also known as MAP2K5, plays a crucial role in regulating the ERK5 signaling pathway, which is essential for various cellular processes such as proliferation, differentiation, and survival. MEK-5 is primarily located in the cytoplasm and activates in response to various extracellular signals, leading to nuclear translocation where MEK-5 influences gene expression. This cytoplasmic-to-nuclear movement enables MEK-5 to interact with downstream targets, including ERK5, thereby modulating critical signaling cascades that impact cell fate decisions. Understanding MEK-5 function and localization helps elucidate its role in physiological and pathological processes, including cancer progression and neurodegenerative diseases.

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MEK-5 Antibody (E-3) References:

1. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
2. MEK5 and ERK5 are localized in the nuclei of resting as well as stimulated cells, while MEKK2 translocates from the cytosol to the nucleus upon stimulation.  |  Raviv, Z., et al. 2004. J Cell Sci. 117: 1773-84. PMID: 15075238
3. Stat3 upregulates MEK5 expression in human breast cancer cells.  |  Song, H., et al. 2004. Oncogene. 23: 8301-9. PMID: 15378007
4. Aberrant expression of extracellular signal-regulated kinase 5 in human prostate cancer.  |  McCracken, SR., et al. 2008. Oncogene. 27: 2978-88. PMID: 18071319
5. Targeted Avenues for Cancer Treatment: The MEK5-ERK5 Signaling Pathway.  |  Pereira, DM. and Rodrigues, CMP. 2020. Trends Mol Med. 26: 394-407. PMID: 32277933
6. MEK5-ERK5 Axis Promotes Self-renewal and Tumorigenicity of Glioma Stem Cells.  |  Fukasawa, K., et al. 2023. Cancer Res Commun. 3: 148-159. PMID: 36968222
7. Pathophysiological Impact of the MEK5/ERK5 Pathway in Oxidative Stress.  |  Tusa, I., et al. 2023. Cells. 12: PMID: 37190064
8. Components of a new human protein kinase signal transduction pathway.  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
9. Independent human MAP-kinase signal transduction pathways defined by MEK and MKK isoforms.  |  Dérijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
10. Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2.  |  Wu, J., et al. 1993. Mol Cell Biol. 13: 4539-48. PMID: 8393135
11. Characterization of the structure and function of a novel MAP kinase kinase (MKK6).  |  Han, J., et al. 1996. J Biol Chem. 271: 2886-91. PMID: 8621675
12. Characterization of the structure and function of a new mitogen-activated protein kinase (p38beta).  |  Jiang, Y., et al. 1996. J Biol Chem. 271: 17920-6. PMID: 8663524

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MEK-5 Antibody (E-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-MEK-5 Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MEK-5 Antibody (E-3) Specifications

Alternate Product Name: MEK-5

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 351-444

Epitope Target: MEK-5

Epitope Species: human

Product Reactivity/Detection: MEK-5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for MEK-5 Antibody (E-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin