MEK-3/6 Antibody (E-3): sc-137106

MEK-3/6 Antibody (E-3) is a mouse monoclonal IgG2a antibody that detects MEK-3/6 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MEK-3, also known as MKK-3, plays a crucial role in the activation of the p38 MAP kinase pathway, which is essential for cellular responses to stress, inflammation, and apoptosis. MEK-3 is primarily located in the cytoplasm, where MEK-3 acts as a key regulator in the signaling cascade that mediates these critical cellular processes. Proper functioning of MEK-3 is vital, as dysregulation can lead to various diseases, including cancer and inflammatory disorders. By phosphorylating p38 MAP kinase, MEK-3 influences downstream targets that control gene expression and cell survival, making MEK-3 an important focus for research in therapeutic interventions. Anti-MEK-3/6 antibody (E-3) is an invaluable tool for researchers aiming to explore the intricate signaling pathways involving p38 MAP kinase and its implications in health and disease.

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MEK-3/6 Antibody (E-3) References:

1. MEK6 regulates human involucrin gene expression via a p38alpha - and p38delta -dependent mechanism.  |  Dashti, SR., et al. 2001. J Biol Chem. 276: 27214-20. PMID: 11454875
2. Tissue inhibitor of metalloproteinase-1 promotes hematopoietic differentiation via caspase-3 upstream the MEKK1/MEK6/p38alpha pathway.  |  Dassé, E., et al. 2007. Leukemia. 21: 595-603. PMID: 17301822
3. Cytokine activation of p38 mitogen-activated protein kinase and apoptosis is opposed by alpha-4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3.  |  Prickett, TD. and Brautigan, DL. 2007. Mol Cell Biol. 27: 4217-27. PMID: 17438131
4. The structure of the MAP2K MEK6 reveals an autoinhibitory dimer.  |  Min, X., et al. 2009. Structure. 17: 96-104. PMID: 19141286
5. Molecular Changes Involving MEK3-p38 MAPK Activation in Chronic Masticatory Myalgia.  |  Meng, H., et al. 2016. J Dent Res. 95: 1169-75. PMID: 27418173
6. MTA2 silencing attenuates the metastatic potential of cervical cancer cells by inhibiting AP1-mediated MMP12 expression via the ASK1/MEK3/p38/YB1 axis.  |  Lin, CL., et al. 2021. Cell Death Dis. 12: 451. PMID: 33958583
7. Quadruple and Truncated MEK3 Mutants Identified from Acute Lymphoblastic Leukemia Promote Degradation and Enhance Proliferation.  |  Ayala-Marin, YM., et al. 2021. Int J Mol Sci. 22: PMID: 34830095
8. PPM1G promotes the progression of lung adenocarcinoma by inhibiting p38 activation via dephosphorylation of MEK6.  |  Chen, J., et al. 2023. Carcinogenesis. 44: 93-104. PMID: 36349938
9. Cloning and characterization of MEK6, a novel member of the mitogen-activated protein kinase kinase cascade.  |  Stein, B., et al. 1996. J Biol Chem. 271: 11427-33. PMID: 8626699
10. Chromosomal localization of four MAPK signaling cascade genes: MEK1, MEK3, MEK4 and MEKK5.  |  Rampoldi, L., et al. 1997. Cytogenet Cell Genet. 78: 301-3. PMID: 9465908

The preferred p-MEK-3/6 antibody is p-MEK-3/6 Antibody (B-9): sc-8407.