Meis1 Double Nickase Plasmid (h): sc-401481-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Meis1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Meis1 Double Nickase Plasmid (h) and Meis1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MEIS1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Meis1/2/3 Antibody (9.2.7): sc-101850
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Meis1 Double Nickase Plasmid (h) | sc-401481-NIC | 20 µg | $410.00 | |||
Meis1 Double Nickase Plasmid (h2) | sc-401481-NIC-2 | 20 µg | $410.00 |
MEIS1 encodes a TALE-class homeobox transcription factor that partners with PBX and HOX proteins to control lineage-specific gene expression programs during development and adult tissue homeostasis. In hematopoietic cells, MEIS1 contributes to transcriptional networks that regulate stem/progenitor maintenance, cell-cycle control, and differentiation, and it interfaces with pathways governing chromatin state and enhancer activity. Dysregulated MEIS1 expression or enhancer perturbation has been linked to leukemogenesis, including MLL-rearranged acute leukemias, where it can cooperate with HOXA cluster programs. MEIS1 is also implicated in cardiovascular and neurodevelopmental processes, making it a useful node for studying transcriptional regulation across multiple organ systems.
Meis1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MEIS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MEIS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MEIS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MEIS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.