megalin/LRP2 Antibody (G-9): sc-515750

Megalin Antibody (G-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects megalin protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Megalin (G-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Megalin, also known as GP330, is a member of the low-density lipoprotein receptor gene family and is characterized by a unique structure that includes a cluster of cysteine-rich class A repeats, epidermal growth factor-like repeats, YWTD repeats, and an O-linked sugar domain. This intricate structure mediates the endocytosis of various ligands, including apolipoprotein J (also known as Clusterin), which plays a significant role in the clearance of beta-amyloid peptides associated with Alzheimer′s disease. Megalin is predominantly located on the apical membrane of epithelial cells, particularly in the proximal tubules of the kidney, the intestine, and ependymal cells, where megalin is essential for the reabsorption of vital molecules such as vitamin B12. The receptor-associated protein (RAP), a molecular chaperone, facilitates proper folding and trafficking of megalin by preventing the uptake of all known ligands, ensuring effective function in cellular processes. Megalin serves as an antigenic determinant in Heymann nephritis in rats, highlighting megalin′s importance in renal physiology and pathology.

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megalin/LRP2 Antibody (G-9) References:

1. Reeler/Disabled-like disruption of neuronal migration in knockout mice lacking the VLDL receptor and ApoE receptor 2.  |  Trommsdorff, M., et al. 1999. Cell. 97: 689-701. PMID: 10380922
2. Functional domains of the very low density lipoprotein receptor: molecular analysis of ligand binding and acid-dependent ligand dissociation mechanisms.  |  Mikhailenko, I., et al. 1999. J Cell Sci. 112 (Pt 19): 3269-81. PMID: 10504332
3. Structural, biochemical and signaling properties of the low-density lipoprotein receptor gene family.  |  Hussain, MM. 2001. Front Biosci. 6: D417-28. PMID: 11229872
4. Role of rap in the biogenesis of lipoprotein receptors.  |  Bu, G. and Marzolo, MP. 2000. Trends Cardiovasc Med. 10: 148-55. PMID: 11239794
5. Megalin and cubilin: synergistic endocytic receptors in renal proximal tubule.  |  Christensen, EI. and Birn, H. 2001. Am J Physiol Renal Physiol. 280: F562-73. PMID: 11249847
6. The membrane-associated guanylate kinase protein MAGI-1 binds megalin and is present in glomerular podocytes.  |  Patrie, KM., et al. 2001. J Am Soc Nephrol. 12: 667-677. PMID: 11274227
7. Urinary megalin in association with progression factors of diabetic nephropathy.  |  Akour, A., et al. 2019. Bratisl Lek Listy. 120: 532-535. PMID: 31602990
8. In vivo real-time imaging reveals megalin as the aminoglycoside gentamicin transporter into cochlea whose inhibition is otoprotective.  |  Kim, J. and Ricci, AJ. 2022. Proc Natl Acad Sci U S A. 119: PMID: 35197290
9. Development of sandwich enzyme-linked immunosorbent assays quantifying mouse urinary megalin, a novel proximal tubular biomarker.  |  Sofuku, R., et al. 2024. J Immunol Methods. 534: 113763. PMID: 39374646
10. Identification of glycoprotein 330 as an endocytic receptor for apolipoprotein J/clusterin.  |  Kounnas, MZ., et al. 1995. J Biol Chem. 270: 13070-5. PMID: 7768901
11. Interaction of apolipoprotein J-amyloid beta-peptide complex with low density lipoprotein receptor-related protein-2/megalin. A mechanism to prevent pathological accumulation of amyloid beta-peptide.  |  Hammad, SM., et al. 1997. J Biol Chem. 272: 18644-9. PMID: 9228033
12. Receptors of the low density lipoprotein (LDL) receptor family in man. Multiple functions of the large family members via interaction with complex ligands.  |  Gliemann, J. 1998. Biol Chem. 379: 951-64. PMID: 9792428

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-megalin/LRP2 Antibody (G-9) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-megalin/LRP2 Antibody (G-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

megalin/LRP2 Antibody (G-9) Specifications

Alternate Product Name: Lrp2

Product Clone Name: G-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 4581-4604

Epitope Target: megalin

Epitope Species: rat

Product Reactivity/Detection: megalin

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for megalin/LRP2 Antibody (G-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred megalin/LRP2 antibody is megalin/LRP2 Antibody (H-10): sc-515772.