Anti-megalin/LRP2 Antibody (G-9) recommended for detection of megalin of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA

Anti-megalin/LRP2 Antibody (G-9): sc-515750

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Datasheets

    • Anti-megalin/LRP2 Antibody (G-9) is a mouse monoclonal IgG1 κ megalin/LRP2 antibody, cited in 5 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 4581-4604 within a C-terminal cytoplasmic domain of megalin of rat origin
    • Anti-megalin/LRP2 Antibody (G-9) is recommended for detection of megalin of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-megalin/LRP2 Antibody (G-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of megalin/LRP2 (G-9): sc-515750.
    • m-IgG1 BP-HRP is the preferred secondary detection reagent for megalin/LRP2 Antibody (G-9) for WB and IHC(P) applications. This reagent is now offered in a bundle with megalin/LRP2 Antibody (G-9) (see ordering information below).
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Hi, I have some questions in regards to this reply "We used a 3-8% gradient gel to obtain the Western blot data image shown for our megalin Antibody (G-9): sc-515750." What kind of stacking gel was used? And what were the transfer conditions? Thanks

Asked by: Mims
Thank you for your question. We use a 4% stacking gel. The conditions for the transfer depends on the electroblotting apparatus used, so please consult your manufacturer´s protocols. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-10-12

How can I get a sample of megalin Antibody (G-9): sc-515750 ?

Asked by: Sayed
Thank you for your question. We are currently offering a free 10 µg sample of this megalin Antibody (G-9): sc-515750. You may contact us directly, or our authorized distributor in Egypt to request a sample. For direct support, please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat. Alternatively, you can find the contact information for our distributor using the link below. https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support
Date published: 2017-08-22

What kind of gel was the sample image representing? Any differences between megalin Antibody (G-9) and megalin Antibody (H-10)? Have these mAbs been tested with mouse samples, in particular for mouse brains (WB and/or IHC)?

Asked by: Mtrf
Thank you for your question. We used a 3-8% gradient gel to obtain the Western blot data image shown for our megalin Antibody (G-9): sc-515750. We do offer two different clones for megalin, (G-9) and (H-10). Both antibodies are raised against the same immunogen corresponding to amino acids 4581-4604 of megalin of rat origin. At this time, the (H-10) clone has been confirmed to be reactive with mouse, rat and human samples. And the (G-9) clone has been confirmed to be reactive with mouse and rat samples only. Both antibodies have published IHC(P) data in mouse kidney tissues.
Answered by: Technical Support
Date published: 2017-03-21

Can megalin (G-9): sc-515750 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, megalin (G-9): sc-515750 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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