Med6 Antibody (1D3): sc-134384
- Med6 Antibody (1D3) is a mouse monoclonal IgG2b κ provided at 100 µg/ml
- raised against recombinant Med6 protein of human origin
- recommended for detection of Med6 of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Med6 Antibody (1D3). This work is in progress.
Med6 Antibody (1D3) is a mouse monoclonal IgG2b antibody that detects Med6 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Med6 plays a crucial role in the regulation of transcription by acting as a coactivator within the high molecular weight coactivating complexes, specifically the SMCC (SRB and MED protein cofactor complex). Med6 (1D3) antibody recognizes components essential for mediating signals between transcriptional activators and RNA polymerase II, thereby enhancing gene-specific activation or repression. The interaction of Med6 with other components of the SMCC, including Srb7 and Med7, is vital for the proper functioning of the transcription machinery, as Med6 facilitates the recruitment of coactivator proteins from the TRAP and DRIP complexes, which are necessary for steroid receptor-dependent transcriptional activation. Furthermore, Med6′s ability to associate with positive cofactor 4 (PC4) highlights its multifaceted role in transcription regulation, as Med6 can also repress basal transcription independently of RNA polymerase II activity. Anti-Med6 antibody (1D3) serves as an invaluable tool for researchers studying the intricate mechanisms of gene expression and the regulatory networks that govern cellular function.
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Med6 Antibody (1D3) References:
- Bioanalytical method development and validation of memantine in human plasma by high performance liquid chromatography with tandem mass spectrometry: application to bioequivalence study. | Konda, RK., et al. 2012. J Anal Methods Chem. 2012: 101249. PMID: 22567548
- Characterization of the influence of mediator complex in HIV-1 transcription. | Ruiz, A., et al. 2014. J Biol Chem. 289: 27665-76. PMID: 25100719
- ABCB1 inhibition provides a novel therapeutic target to block TWIST1-induced migration in medulloblastoma. | Nasir, A., et al. 2021. Neurooncol Adv. 3: vdab030. PMID: 33948561
- Identification of Therapeutic Targets and Prognostic Biomarkers among Genes from the Mediator Complex Family in the Hepatocellular Carcinoma Tumour-Immune Microenvironment. | Tan, W., et al. 2022. Comput Math Methods Med. 2022: 2021613. PMID: 35069777
- Identification of diagnostic genes for both Alzheimer's disease and Metabolic syndrome by the machine learning algorithm. | Li, J., et al. 2022. Front Immunol. 13: 1037318. PMID: 36405716
- Yeast PIC-Mediator structure with RNA polymerase II C-terminal domain. | Schilbach, S., et al. 2023. Proc Natl Acad Sci U S A. 120: e2220542120. PMID: 37014863
- Regulation of fungal raw-starch-degrading enzyme production depends on transcription factor phosphorylation and recruitment of the Mediator complex. | Ning, YN., et al. 2023. Commun Biol. 6: 1032. PMID: 37828083
- A new strategy for screening novel functional genes involved in reduction of lipid droplet accumulation. | Maruyama, R., et al. 2024. Biofactors. 50: 467-476. PMID: 37983968
- Requirement for a functional interaction between mediator components Med6 and Srb4 in RNA polymerase II transcription. | Lee, YC. and Kim, YJ. 1998. Mol Cell Biol. 18: 5364-70. PMID: 9710620
- Activator-specific requirement of yeast mediator proteins for RNA polymerase II transcriptional activation. | Han, SJ., et al. 1999. Mol Cell Biol. 19: 979-88. PMID: 9891034
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Med6 Antibody (1D3) | sc-134384 | 100 µg/ml | $339.00 |