Med29 Antibody (B-1): sc-393800
- Med29 Antibody (B-1) is a mouse monoclonal IgG2b κ Med29 antibody provided at 200 µg/ml
- raised against amino acids 63-221 mapping at the C-terminus of Med29 of human origin
- Med29 Antibody (B-1) is recommended for detection of Med29 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Med29 Antibody (B-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for Med29 Antibody (B-1) for WB applications. This reagent is now offered in a bundle with Med29 Antibody (B-1) (see ordering information below).
Med29 Antibody (B-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects Med29 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Med29 (B-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Med29, also known as mediator complex subunit 29 or IXL (intersex-like), is a crucial component of the mediator complex, which plays a vital role in regulating transcription by serving as a bridge between transcriptional activators and RNA polymerase II. This 200 amino acid nuclear protein is widely expressed in both embryonic and adult tissues, highlighting Med29′s importance in various biological processes. Notably, Med29 has been identified as a novel amplification target gene in pancreatic cancer, underscoring Med29′s potential significance in cancer research and therapy. The gene encoding Med29 is located on human chromosome 19q13.2, a region known for its high gene density, containing approximately 1,400 genes within over 63 million bases. The intricate interactions and regulatory mechanisms involving Med29 are essential for understanding transcriptional control and its implications in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Med29 Antibody (B-1) References:
- A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex. | Sato, S., et al. 2003. J Biol Chem. 278: 49671-4. PMID: 14576168
- A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. | Tomomori-Sato, C., et al. 2004. J Biol Chem. 279: 5846-51. PMID: 14638676
- A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. | Sato, S., et al. 2004. Mol Cell. 14: 685-91. PMID: 15175163
- IXL, a new subunit of the mammalian Mediator complex, functions as a transcriptional suppressor. | Wang, Y., et al. 2004. Biochem Biophys Res Commun. 325: 1330-8. PMID: 15555573
- MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. | Zhang, X., et al. 2005. Mol Cell. 19: 89-100. PMID: 15989967
- Intersex-like (IXL) is a cell survival regulator in pancreatic cancer with 19q13 amplification. | Kuuselo, R., et al. 2007. Cancer Res. 67: 1943-9. PMID: 17332321
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Med29 Antibody (B-1) | sc-393800 | 200 µg/ml | $322.00 | |||
Med29 Antibody (B-1): m-IgG2b BP-HRP Bundle | sc-548783 | 200 µg Ab; 10 µg BP | $361.00 | |||
Med29 Antibody (B-1) AC | sc-393800 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Med29 Antibody (B-1) HRP | sc-393800 HRP | 200 µg/ml | $322.00 | |||
Med29 Antibody (B-1) FITC | sc-393800 FITC | 200 µg/ml | $336.00 | |||
Med29 Antibody (B-1) PE | sc-393800 PE | 200 µg/ml | $349.00 | |||
Med29 Antibody (B-1) Alexa Fluor® 488 | sc-393800 AF488 | 200 µg/ml | $364.00 | |||
Med29 Antibody (B-1) Alexa Fluor® 546 | sc-393800 AF546 | 200 µg/ml | $364.00 | |||
Med29 Antibody (B-1) Alexa Fluor® 594 | sc-393800 AF594 | 200 µg/ml | $364.00 | |||
Med29 Antibody (B-1) Alexa Fluor® 647 | sc-393800 AF647 | 200 µg/ml | $364.00 | |||
Med29 Antibody (B-1) Alexa Fluor® 680 | sc-393800 AF680 | 200 µg/ml | $364.00 | |||
Med29 Antibody (B-1) Alexa Fluor® 790 | sc-393800 AF790 | 200 µg/ml | $364.00 |