Med10 Double Nickase Plasmid (h): sc-407658-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Med10 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Med10 Double Nickase Plasmid (h) and Med10 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MED10. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Med10 Antibody (C-2): sc-393450
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Med10 Double Nickase Plasmid (h) | sc-407658-NIC | 20 µg | $410.00 | |||
Med10 Double Nickase Plasmid (h2) | sc-407658-NIC-2 | 20 µg | $410.00 |
MED10 encodes a subunit of the Mediator complex, a central coordinator of RNA polymerase II–dependent transcription that links sequence-specific transcription factors to the core transcriptional machinery. Med10 contributes to assembly and stability of Mediator modules that regulate transcription initiation and signal-dependent gene expression programs. Through these roles, MED10 influences broad cellular processes including proliferation, differentiation, and stress-responsive transcriptional networks. Dysregulation of Mediator components is associated with altered transcriptional control in developmental disorders and cancer-relevant gene expression signatures, making MED10 a useful node for mechanistic studies of transcriptional regulation.
Med10 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MED10 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MED10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MED10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MED10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.