Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MeCP2 Double Nickase Plasmid (h): sc-401228-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MeCP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MeCP2 Double Nickase Plasmid (h) and MeCP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MECP2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MeCP2 Antibody (G-6): sc-137070
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MeCP2 Double Nickase Plasmid (h) | sc-401228-NIC | 20 µg | $410.00 | |||
MeCP2 Double Nickase Plasmid (h2) | sc-401228-NIC-2 | 20 µg | $410.00 |
MECP2 encodes methyl-CpG–binding protein 2 (MeCP2), a chromatin-associated regulator that interprets DNA methylation and modulates transcriptional programs in a context-dependent manner. MeCP2 binds methylated CpG dinucleotides and interacts with corepressor and chromatin remodeling complexes, influencing histone modification, chromatin compaction, and RNA polymerase II–mediated gene expression. Through these epigenetic mechanisms, MeCP2 contributes to neuronal maturation, synaptic function, and activity-dependent transcriptional responses. Dysregulation of MECP2 is strongly linked to neurodevelopmental disorders, and it is frequently studied in models of transcriptional control, chromatin biology, and neuronal gene networks.
MeCP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MECP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MECP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MECP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MECP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.