MeCP2 Antibody (G-6): sc-137070

MeCP2 Antibody (G-6) is a mouse monoclonal IgG3 kappa light chain antibody that detects MeCP2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-MeCP2 antibody (G-6) is available as a non-conjugated format. MeCP2 plays a crucial role in regulating gene expression through binding to methylated DNA, which is essential for normal neurological function. Mutations in MeCP2 are linked to Rett syndrome, a severe neurodevelopmental disorder primarily affecting females, highlighting MeCP2′s importance in maintaining cognitive and motor functions. MeCP2 is predominantly located in the nucleus, where MeCP2 interacts with methylated DNA and various corepressor complexes, including mSin3A and histone deacetylases, to mediate transcriptional repression. This nuclear localization enables MeCP2 to effectively regulate gene expression in response to DNA methylation changes, which are critical for neuronal development and plasticity. MeCP2′s ability to bind tightly to methylated regions of the genome underscores MeCP2′s significance in epigenetic regulation and potential impact on various neurological disorders when dysregulated.

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MeCP2 Antibody (G-6) References:

1. Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes.  |  Hendrich, B., et al. 1999. Mamm Genome. 10: 906-12. PMID: 10441743
2. Methylation-mediated transcriptional silencing in euchromatin by methyl-CpG binding protein MBD1 isoforms.  |  Fujita, N., et al. 1999. Mol Cell Biol. 19: 6415-26. PMID: 10454587
3. MECP2 deletions and genotype-phenotype correlation in Rett syndrome.  |  Scala, E., et al. 2007. Am J Med Genet A. 143A: 2775-84. PMID: 17968969
4. Integrated epigenomic analyses of neuronal MeCP2 reveal a role for long-range interaction with active genes.  |  Yasui, DH., et al. 2007. Proc Natl Acad Sci U S A. 104: 19416-21. PMID: 18042715
5. MeCP2 mutation causes distinguishable phases of acute and chronic defects in synaptogenesis and maintenance, respectively.  |  Palmer, A., et al. 2008. Mol Cell Neurosci. 37: 794-807. PMID: 18295506
6. Common variants within MECP2 confer risk of systemic lupus erythematosus.  |  Sawalha, AH., et al. 2008. PLoS One. 3: e1727. PMID: 18320046
7. DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein.  |  Boyes, J. and Bird, A. 1991. Cell. 64: 1123-34. PMID: 2004419
8. Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex.  |  Nan, X., et al. 1998. Nature. 393: 386-9. PMID: 9620804
9. Identification and characterization of a family of mammalian methyl-CpG binding proteins.  |  Hendrich, B. and Bird, A. 1998. Mol Cell Biol. 18: 6538-47. PMID: 9774669

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MeCP2 Antibody (G-6) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-MeCP2 Antibody (G-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MeCP2 Antibody (G-6) Specifications

Alternate Product Name: Methyl-CpG Binding Protein 2

Product Clone Name: G-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 187-486

Epitope Target: MeCP2

Epitope Species: human

Product Reactivity/Detection: MeCP2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for MeCP2 Antibody (G-6): X: TransCruz Reagent